Fluorescent probe based on N-doped carbon dots for the detection of intracellular pH and glutathione.
Fluorescent probe based on N-doped carbon dots for the detection of intracellular pH and glutathione.
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基于氮掺杂碳点的荧光探针用于检测细胞内pH和谷胱甘肽
作者：
碳量子点（CD）作为荧光探针已被广泛用于检测生物标志物，然而，通常需要对CD进行繁琐的表面修饰以实现相对良好的检测能力。本文以三乙烯四胺（TETA）和间苯二胺（m-PD）为原料，采用一步水热法合成了氮掺杂碳量子点（N-CD）。当pH从3增加到11时，N-CD的荧光强度逐渐降低。此外，它对5-8的生理pH范围显示出线性响应。Au 3+被N-CD表面上的氨基还原以生成金纳米颗粒（AuNPs），导致N-CD的荧光猝灭。如果随后加入谷胱甘肽（GSH），则N-CD的荧光被恢复。N-CD的荧光强度与GSH浓度在50-400 μM范围内呈线性关系，检测限（LOD）为7.83 μM。荧光探针用于使用pH区分癌细胞和正常细胞，并评估细胞内GSH。本工作拓展了CD在多组分检测中的应用，为细胞内pH和GSH的检测提供了一种简便的荧光探针。
Carbon dots (CDs) as fluorescent probes have been widely exploited to detect biomarkers, however, tedious surface modification of CDs is generally required to achieve a relatively good detection ability. Here, we synthesized N-doped carbon dots (N-CDs) from triethylenetetramine (TETA) and m-phenylenediamine (m-PD) using a one-step hydrothermal method. When the pH increases from 3 to 11, the fluorescence intensity of the N-CDs gradually decreases. Furthermore, it displays a linear response to the physiological pH range of 5–8. Au3+ is reduced by amino groups on the surface of N-CDs to generate gold nanoparticles (AuNPs), causing fluorescence quenching of the N-CDs. If glutathione (GSH) is then added, the fluorescence of the N-CDs is recovered. The fluorescence intensity of the N-CDs is linearly correlated with the GSH concentration in the range of 50–400 μM with a limit of detection (LOD) of 7.83 μM. The fluorescence probe was used to distinguish cancer cells from normal cells using pH and to evaluate intracellular GSH. This work expands the application of CDs in multicomponent detection and provides a facile fluorescent probe for the detection of intracellular pH and GSH.
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影响因子：
4.1
作者：
Fiuza, Thiago;Gomide, Guilherme;Depeyrot, Jerome
通讯作者：
Depeyrot, Jerome
影响因子：
12.6
作者：
Gu, Jiangjiang;Hu, Donghua;Xi, Kai
通讯作者：
Xi, Kai
多功能 N,S 共掺杂碳量子点，具有 pH 和热依赖性可切换荧光特性以及谷胱甘肽的高选择性检测
影响因子：
10.9
作者：
Song, Zhongqian;Quan, Fengyu;Liu, Jingquan
通讯作者：
Liu, Jingquan
影响因子：
15
作者：
Lee, Jun Han;Lim, Chang Su;Cho, Bong Rae
通讯作者：
Cho, Bong Rae
影响因子：
8.4
作者：
Chu, Binyang;Qi, Tingting;Qian, Zhiyong
通讯作者：
Qian, Zhiyong