Gas chromatography-mass spectrometry-based metabolic profiling of cerebrospinal fluid from epileptic dogs.
Gas chromatography-mass spectrometry-based metabolic profiling of cerebrospinal fluid from epileptic dogs.
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DOI：
10.1292/jvms.13-0520
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发表时间：
2014-04
期刊：
影响因子：
--
通讯作者：
Takenaka S
中科院分区：
文献类型：
--
作者：
Hasegawa T;Sumita M;Horitani Y;Tamai R;Tanaka K;Komori M;Takenaka S
癫痫是一种常见的神经系统疾病，伴有癫痫发作，但兽医诊所的诊断方法仍然有限。脑脊液（CSF）是一种用于兽医诊断的体液。在这项研究中，我们使用基于气相色谱-质谱（GC-MS）的脑脊液代谢分析和多变量数据分析来探索犬癫痫诊断生物标志物。特发性癫痫受试者的概况与健康对照和症状性癫痫受试者的概况显着不同。在 60 种已鉴定的代谢物中，三组中有 20 种代谢物的水平存在显着差异。谷氨酸在特发性癫痫中显着增加，并且包括抗坏血酸在内的一些代谢物在两种形式的癫痫中都发生了变化。这些发现表明，特发性癫痫和症状性癫痫之间的脑脊液代谢特征不同，脑脊液中的谷氨酸和抗坏血酸等代谢物可能有助于犬癫痫的诊断。
Epilepsy is a common neurological disorder with seizures, but diagnostic approaches in veterinary clinics remain limited. Cerebrospinal fluid (CSF) is a body fluid used for diagnosis in veterinary medicine. In this study, we explored canine epilepsy diagnostic biomarkers using gas chromatography-mass spectrometry (GC-MS)-based metabolic profiling of CSF and multivariate data analysis. Profiles for subjects with idiopathic epilepsy differed significantly from those of healthy controls and subjects with symptomatic epilepsy. Among 60 identified metabolites, the levels of 20 differed significantly among the three groups. Glutamic acid was significantly increased in idiopathic epilepsy, and some metabolites including ascorbic acid were changed in both forms of epilepsy. These findings show that metabolic profiles of CSF differ between idiopathic and symptomatic epilepsy and that metabolites including glutamic acid and ascorbic acid in CSF may be useful for diagnosis of canine epilepsy.
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