Collagen scaffolds with controlled insulin release and controlled pore structure for cartilage tissue engineering.
Collagen scaffolds with controlled insulin release and controlled pore structure for cartilage tissue engineering.
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DOI：
10.1155/2014/623805
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发表时间：
2014
影响因子：
--
通讯作者：
Chen G
中科院分区：
文献类型：
--
作者：
Nanda HS;Chen S;Zhang Q;Kawazoe N;Chen G
生长因子和营养物从多孔支架的受控和局部释放对于维持细胞存活、增殖和促进组织再生是重要的。本研究的目的是设计一种可控释放多孔胶原微珠杂化支架，具有可控的孔结构，能够释放胰岛素用于软骨组织再生。采用冷冻干燥法将负载胰岛素的PLGA微球与胶原蛋白杂交，制备胶原-微球杂交支架。通过使用直径范围为150-250 μm的预先制备的冰颗粒来控制杂化支架的孔结构。杂化支架具有可控的孔结构，其孔径相当于冰颗粒，并且具有良好的互连性。微珠在整个孔壁中显示出均匀的空间分布。在体外胰岛素释放曲线从混合支架表现出零级释放动力学长达4周的时间没有初始爆发释放。牛关节软骨细胞在杂交支架上的培养显示出高的胰岛素生物活性。该混合支架有利于细胞接种和空间细胞分布，并促进细胞增殖。
Controlled and local release of growth factors and nutrients from porous scaffolds is important for maintenance of cell survival, proliferation, and promotion of tissue regeneration. The purpose of the present research was to design a controlled release porous collagen-microbead hybrid scaffold with controlled pore structure capable of releasing insulin for application to cartilage tissue regeneration. Collagen-microbead hybrid scaffold was prepared by hybridization of insulin loaded PLGA microbeads with collagen using a freeze-drying technique. The pore structure of the hybrid scaffold was controlled by using preprepared ice particulates having a diameter range of 150–250 μm. Hybrid scaffold had a controlled pore structure with pore size equivalent to ice particulates and good interconnection. The microbeads showed an even spatial distribution throughout the pore walls. In vitro insulin release profile from the hybrid scaffold exhibited a zero order release kinetics up to a period of 4 weeks without initial burst release. Culture of bovine articular chondrocytes in the hybrid scaffold demonstrated high bioactivity of the released insulin. The hybrid scaffold facilitated cell seeding and spatial cell distribution and promoted cell proliferation.
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DOI：
10.1002/jbm.b.31586
发表时间：
2010-05-01
影响因子：
3.4
作者：
Ko, Young-Gwang;Kawazoe, Naoki;Chen, Guoping
通讯作者：
Chen, Guoping
影响因子：
14
作者：
Li, WJ;Tuli, R;Tuan, RS
通讯作者：
Tuan, RS
影响因子：
1.7
作者：
Borzacchiello, A.;Gloria, A.;Ambrosio, L.
通讯作者：
Ambrosio, L.
影响因子：
9.7
作者：
Andreas, Kristin;Zehbe, Rolf;Ringe, Jochen
通讯作者：
Ringe, Jochen
影响因子：
14
作者：
Lu, Hongxu;Ko, Young-Gwang;Chen, Guoping
通讯作者：
Chen, Guoping