Near infrared fluorescent optical imaging for nodal staging.
Near infrared fluorescent optical imaging for nodal staging.
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DOI：
10.1117/1.2953498
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发表时间：
2008-07
影响因子：
3.5
通讯作者：
Sevick-Muraca EM
中科院分区：
文献类型：
--
作者：
Sampath L;Wang W;Sevick-Muraca EM
目前评估癌症患者淋巴结转移的技术包括施用非特异性放射性胶体后的淋巴结造影术，以定位和切除淋巴结，用于对所携带的癌细胞进行病理学检查。涉及皮内或皮下注射基于抗体的核成像剂的临床试验已经证明了癌症阳性淋巴结的靶向特异性分子成像的可行性。采用近红外（NIR）光学成像评估疾病的基础是由最近的工作证明，显示在小鼠，猪和人类的功能性淋巴成像。我们回顾了基于抗体的免疫淋巴显像，重点是使用曲妥珠单抗（或赫赛汀）靶向在某些乳腺癌中过表达的人表皮生长因子受体2（HER 2）。具体而言，我们回顾了我们实验室的体外和临床前成像数据，这些数据显示了双标记试剂（111 In-DTPA）n-曲妥珠单抗-（IRDye 800）m如何利用NIR荧光染料IRDye 800 CW提供的高光子计数和γ发射体铟-111的放射性信号，用于可能检测淋巴结中的HER 2转移。我们发现，（111铟-DTPA）n-曲妥珠单抗-（IRDye 800）m从小鼠腋窝淋巴结的积累和清除发生48小时后，皮内注射到足背方面。对正常的、癌症阴性的淋巴结的长清除时间的要求对具有有限半衰期但不妨碍NIR光学成像的核成像剂提出了挑战。
Current techniques to assess lymph node metastases in cancer patients include lymphoscintigraphy after administration of a nonspecific radiocolloid in order to locate and resect lymph nodes for pathological examination of harbored cancer cells. Clinical trials involving intradermal or subcutaneous injection of antibody-based nuclear imaging agents have demonstrated the feasibility for target-specific, molecular imaging of cancer-positive lymph nodes. The basis for employing near-infrared (NIR) optical imaging for assessing disease is evidenced by recent work showing functional lymph imaging in mice, swine, and humans. We review antibody-based immunolymphoscintigraphy with an emphasis on the use of trastuzumab (or Herceptin) to target human epidermal growth factor receptor-2 (HER2) overexpressed in some breast cancers. Specifically, we review in vitro and preclinical imaging data from our laboratory that show how the dual-labeled agent (111In-DTPA)n-trastuzumab-(IRDye800)m utilizes the high photon count provided by an NIR fluorescent dye, IRDye 800CW, and the radioactive signal from a gamma emitter, Indium-111, for possible detection of HER2 metastasis in lymph nodes. We show that the accumulation and clearance of (111In-DTPA)n-trastuzumab-(IRDye800)m from the axillary nodes of mice occurs 48 h after intradermal injection into the dorsal aspect of the foot. The requirement for long clearance times from normal, cancer-negative nodes presents challenges for nuclear imaging agents with limited half-lives but does not hamper NIR optical imaging.
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影响因子：
3.7
作者：
Parungo, Cherie P.;Soybel, David I.;Frangioni, John V.
通讯作者：
Frangioni, John V.
DOI：
10.1007/bf01146853
发表时间：
1990-03-01
期刊：
EUROPEAN JOURNAL OF NUCLEAR MEDICINE
影响因子：
--
作者：
ABDELNABI, HH;ORTMANNABI, JA;HALVERSON, C
通讯作者：
HALVERSON, C
影响因子：
3.3
作者：
Artemov, D;Mori, N;Bhujwalla, ZM
通讯作者：
Bhujwalla, ZM
影响因子：
3.8
作者：
Joshi, Amit;Bangerth, Wolfgang;Sevick-Muraca, Eva M.
通讯作者：
Sevick-Muraca, Eva M.
影响因子：
3.8
作者：
Corlu, Alper;Choe, Regine;Yodh, Arjun G.
通讯作者：
Yodh, Arjun G.