Mechanistic study on the inhibition of Staphylococcus epidermidis biofilm by agrC-specific binding polypeptide
Mechanistic study on the inhibition of Staphylococcus epidermidis biofilm by agrC-specific binding polypeptide
复制标题
agrC特异性结合多肽抑制表皮葡萄球菌生物膜的机制研究
DOI：
10.21037/atm.2020.02.84
复制
发表时间：
2020-03
影响因子：
--
通讯作者：
Lianhua Ye
中科院分区：
文献类型：
--
作者：
Bingquan Xiang;Yujie Lei;Ya Chen;Guangqiang Zhao;Yongchun Zhou;Youquan Zhou;Yunchao Huang;Lianhua Ye
背景抗生素的滥用日益严重，开发新的抗菌药物可有效防止细菌耐药性的产生。对表皮葡萄球菌agrC特异性结合多肽抑制S.表皮生物膜的形成可能为新型抗菌药物的开发提供思路。方法S.用不同浓度（0、100、200、400、800和1，600 µg/mL）的agrC特异性结合多肽（N1）和空白（N 0）培养表皮细胞。通过结晶紫染色检测生物膜的形成情况，确定agrC特异性结合多肽的最佳浓度，并测定细菌抑制浓度。在不同时间点（6、12、18、24和30 h），采用XTT法检测细菌活力，采用实时定量聚合酶链反应检测atlE、icaA、fbe和icaR基因的表达。采用硫酸-苯酚法测定多糖细胞间粘附素（PIA）含量。结果S.在用800 μg/mL agrC特异性结合多肽处理后，表皮细胞的生长最低。培养6 h后，agrC特异性结合多肽上调atlE、icaA、fbe和icaR的表达，并增加细菌活力。然而，该多肽下调atlE、icaA、fbe和icaR的表达，并抑制S.表皮生长和PIA形成。尽管agrC特异性结合多肽在18 h后上调atlE、icaA、fbe和icaR的表达，但它们抑制细菌生长和PIA形成。结论agrC特异性结合多肽可下调atlE、icaA、fbe和icaR的表达，抑制S.表皮葡萄球菌生物膜形成能力的抑制作用。表皮其有效浓度为800 µg/mL。
Background Considering the wide-spread misuse of antibiotics, the development of new antibacterial drugs may effectively prevent the emergence of antibiotic resistance in bacteria. The understanding of the mechanism underlying the Staphylococcus epidermidis agrC-specific binding polypeptide-mediated inhibition of S. epidermidis biofilm formation may supply ideas for the development of new antibacterial drugs. Methods S. epidermidis cells were cultured with different concentrations (0, 100, 200, 400, 800, and 1,600 µg/mL) of agrC-specific binding polypeptide (N1) and blank (N0). Crystal violet staining was performed to test the formation of biofilms and to determine the best concentration of agrC-specific binding polypeptides, and the bacterial inhibitory concentration was also determined. At different time points (6, 12, 18, 24, and 30 h), XTT assay was used to measure bacterial viability, and the real-time quantitative polymerase chain reaction was performed to measure the expression of atlE, icaA, fbe, and icaR genes. The sulfuric acid-phenol method was used to determine polysaccharide intercellular adhesin (PIA) levels. Results The biofilm formation ability of S. epidermidis was the lowest after treatment with 800 µg/mL agrC-specific binding polypeptide. After 6 h of culture, agrC-specific binding polypeptide upregulated the expression of atlE, icaA, fbe, and icaR and increased the bacterial viability. However, the polypeptide downregulated the expression of atlE, icaA, fbe, and icaR and inhibited S. epidermidis growth and PIA formation after 12 h of culture. Although agrC-specific binding polypeptide upregulated the expression of atlE, icaA, fbe, and icaR after 18 h, they inhibited bacterial growth and PIA formation. Conclusions Thus, agrC-specific binding polypeptide could downregulate the expression of atlE, icaA, fbe, and icaR and inhibit PIA formation by S. epidermidis after 12 h, demonstrating its transient inhibitory effects on the biofilm formation ability of S. epidermidis. Its effective concentration was 800 µg/mL.
登录
查看更多内容
影响因子：
2.4
作者：
Wolska KI;Grudniak AM;Rudnicka Z;Markowska K
通讯作者：
Markowska K
影响因子：
2
作者：
Ye Lianhua;Huang Yunchao;Guo Fengli
通讯作者：
Guo Fengli
金黄色葡萄球菌自诱导剂 2 群体感应以 icaR 依赖性方式减少生物膜形成
影响因子：
4.2
作者：
Yu D;Zhao L;Xue T;Sun B
通讯作者：
Sun B
影响因子：
5.2
作者：
Sabaté Brescó M;Harris LG;Thompson K;Stanic B;Morgenstern M;O'Mahony L;Richards RG;Moriarty TF
通讯作者：
Moriarty TF
影响因子：
3.2
作者：
Hoang, Tra-My;Zhou, C.;Fey, P. D.
通讯作者：
Fey, P. D.