Amine-functionalized polypyrrole: Inherently cell adhesive conducting polymer.
Amine-functionalized polypyrrole: Inherently cell adhesive conducting polymer.
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DOI：
10.1002/jbm.a.35344
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发表时间：
2015-06
影响因子：
4.9
通讯作者：
Schmidt, Christine E.
中科院分区：
文献类型：
--
作者：
Lee, Jae Y.;Schmidt, Christine E.
导电聚合物是一种新型的生物材料，可以与生物系统进行电通信。在组织工程应用中，导电聚合物已被修饰以促进细胞粘附，从而改善生物材料与细胞/组织之间的相互作用。提高细胞粘附性的传统方法包括用生物分子对导电聚合物进行表面修饰，例如对细胞粘附蛋白和聚阳离子聚合物进行物理吸附，或对其进行化学固定；然而，这些方法需要使用昂贵的生物分子进行额外的多次修饰。在这项研究中，作为一种简单有效的替代这种额外的生物分子处理的方法，我们合成了胺功能化聚吡咯（APPy），它在生理条件下固有地呈现支持细胞粘附的正电荷。与常规聚吡咯（PPy）均聚物相比，合成的聚吡咯具有中等范围的电活性和亲水性表面。在血清和无血清条件下，与PPy均聚物对照相比，APPy对人皮肤成纤维细胞和雪旺细胞的附着能力更强。此外，雪旺细胞在APPy共聚物上的粘附至少与聚l -赖氨酸处理的PPy对照组相似。我们的研究结果表明，胺功能化的导电聚合物衬底将有助于实现良好的细胞粘附和潜在的电刺激各种细胞。此外，存在于导电聚合物上的胺功能可以进一步作为一种新颖而灵活的平台，以化学方式捆绑各种生物活性分子，如生长因子、抗体和化学药物。
Electrically conducting polymers have been recognized as novel biomaterials that can electrically communicate with biological systems. For their tissue engineering applications, conducting polymers have been modified to promote cell adhesion for improved interactions between biomaterials and cells/tissues. Conventional approaches to improve cell adhesion involve the surface modification of conducting polymers with biomolecules, such as physical adsorption of cell adhesive proteins and polycationic polymers, or their chemical immobilization; however, these approaches require additional multiple modification steps with expensive biomolecules. In this study, as a simple and effective alternative to such additional biomolecule treatment, we synthesized amine-functionalized polypyrrole (APPy) that inherently presents cell adhesion-supporting positive charges under physiological conditions. The synthesized APPy provides electrical activity in a moderate range and a hydrophilic surface compared to regular polypyrrole (PPy) homopolymers. Under both serum and serum-free conditions, APPy exhibited superior attachment of human dermal fibroblasts and Schwann cells compared to PPy homopolymer controls. Moreover, Schwann cell adhesion onto the APPy copolymer was at least similar to that on poly-L-lysine treated PPy controls. Our results indicate that amine-functionalized conducting polymer substrates will be useful to achieve good cell adhesion and potentially electrically stimulate various cells. In addition, an amine functionality present on conducting polymers can further serve as a novel and flexible platform to chemically tether various bioactive molecules, such as growth factors, antibodies, and chemical drugs.
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影响因子：
9.7
作者：
Lee, Jae Young;Schmidt, Christine E.
通讯作者：
Schmidt, Christine E.
影响因子：
16.2
作者：
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使用导电聚吡咯/壳聚糖聚合物对雪旺细胞进行电调节
影响因子：
4.9
作者：
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通讯作者：
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作者：
Eisenbarth, E;Meyle, J;Breme, J
通讯作者：
Breme, J
影响因子：
4.9
作者：
Meng, Shiyun;Rouabhia, Mahmoud;Zhang, Ze
通讯作者：
Zhang, Ze