Expression of vesicular glutamate transporters type 1 and 2 in sensory and autonomic neurons innervating the mouse colorectum.
Expression of vesicular glutamate transporters type 1 and 2 in sensory and autonomic neurons innervating the mouse colorectum.
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DOI：
10.1002/cne.22730
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发表时间：
2011-11-01
影响因子：
2.5
通讯作者：
Gebhart, G. F.
中科院分区：
文献类型：
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作者：
Brumovsky, Pablo R.;Robinson, David R.;La, Jun-Ho;Seroogy, Kim B.;Lundgren, Kerstin H.;Albers, Kathryn M.;Kiyatkin, Michael E.;Seal, Rebecca P.;Edwards, Robert H.;Watanabe, Masahiko;Hokfelt, Tomas;Gebhart, G. F.
囊泡谷氨酸转运体（VGLUTs）在各种神经系统中已被广泛研究，但其在内脏感觉和自主神经元中的表达仍有待详细分析。在这里，我们研究了VGLUT类型1和2（VGLUT 1和VGLUT 2，分别）在神经支配小鼠结直肠的神经元。用固蓝逆行追踪BALB/C小鼠支配结肠直肠的腰骶和胸腰背根神经节（DRG）、腰交感链（LSC）和主要盆神经节（MPG）神经元，解剖并进行免疫组织化学处理。包括来自额外的未处理小鼠的组织。使用先前表征的抗VGLUT 1、VGLUT 2和降钙素基因相关肽（CGRP）的抗体。还使用针对VGLUT 1和VGLUT 2的探针进行核糖核酸探针原位杂交。大多数结直肠DRG神经元表达VGLUT 2，并且经常与CGRP共定位。表达VGLUT 1的神经元百分比较小。MPG中VGLUT 2免疫反应（IR）神经元很少。在结直肠的所有层中检测到丰富的VGLUT 2-IR神经; VGLUT 1-IR神经稀疏。一个亚群的肌间神经丛神经元表达VGLUT 2蛋白和mRNA，但VGLUT 1 mRNA检测不到。总之，我们发现1）大多数结直肠DRG神经元表达VGLUT 2，在较小程度上表达VGLUT 1; 2）丰富的VGLUT 2-IR纤维支配结直肠; 3）肌间神经丛神经元亚群表达VGLUT 2。总而言之，我们的数据表明VGLUT 2在结直肠平滑肌能神经传递中的作用，可能影响结直肠的敏感性和运动性。
Vesicular glutamate transporters (VGLUTs) have been extensively studied in various neuronal systems, but their expression in visceral sensory and autonomic neurons remains to be analyzed in detail. Here we studied VGLUTs type 1 and 2 (VGLUT1 and VGLUT2, respectively) in neurons innervating the mouse colorectum. Lumbosacral and thoracolumbar dorsal root ganglion (DRG), lumbar sympathetic chain (LSC), and major pelvic ganglion (MPG) neurons innervating the colorectum of BALB/C mice were retrogradely traced with Fast Blue, dissected, and processed for immunohistochemistry. Tissue from additional naïve mice was included. Previously characterized antibodies against VGLUT1, VGLUT2, and calcitonin gene-related peptide (CGRP) were used. Riboprobe in situ hybridization, using probes against VGLUT1 and VGLUT2, was also performed. Most colorectal DRG neurons expressed VGLUT2 and often colocalized with CGRP. A smaller percentage of neurons expressed VGLUT1. VGLUT2-immunoreactive (IR) neurons in the MPG were rare. Abundant VGLUT2-IR nerves were detected in all layers of the colorectum; VGLUT1-IR nerves were sparse. A subpopulation of myenteric plexus neurons expressed VGLUT2 protein and mRNA, but VGLUT1 mRNA was undetectable. In conclusion, we show 1) that most colorectal DRG neurons express VGLUT2, and to a lesser extent, VGLUT1; 2) abundance of VGLUT2-IR fibers innervating colorectum; and 3) a subpopulation of myenteric plexus neurons expressing VGLUT2. Altogether, our data suggests a role for VGLUT2 in colorectal glutamatergic neurotransmission, potentially influencing colorectal sensitivity and motility.
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影响因子：
2.5
作者：
Boulland, JL;Qureshi, T;Chaudhry, FA
通讯作者：
Chaudhry, FA
影响因子：
2.5
作者：
Alvarez, FJ;Villalba, RM;Schneider, SP
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Schneider, SP
影响因子：
6.2
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通讯作者：
Derouiche, A
影响因子：
29.4
作者：
Brierley, SM;Jones, RCW;Blackshaw, LA
通讯作者：
Blackshaw, LA
影响因子：
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作者：
ABERCROMBIE, M
通讯作者：
ABERCROMBIE, M