Diurnal Variation in Trigeminal Pain Sensitivity in Mice.
Diurnal Variation in Trigeminal Pain Sensitivity in Mice.
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DOI：
10.3389/fnins.2021.703440
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发表时间：
2021
影响因子：
4.3
通讯作者：
Sugimura M
中科院分区：
文献类型：
--
作者：
Niiro A;Ohno SN;Yamagata KA;Yamagata K;Tomita K;Kuramoto E;Oda Y;Nakamura TJ;Nakamura W;Sugimura M
在疼痛和止痛的基础研究中，对时间和昼夜节律干扰的管理是一个极其重要的因素。虽然已知疼痛在一天中会发生变化，但这种昼夜变化背后的机制在很大程度上仍不清楚。在这项研究中，我们假设疼痛传递到中枢神经系统的过程(在收到来自体外的伤害性刺激后)会显示出昼夜差异。10周龄雄性小鼠在严格的12/12小时光/暗循环下至少10天。然后将福尔马林注射到三叉神经第二支区域，并评估疼痛相关行为(PRB)的持续时间。免疫组织化学染色，计数三叉神经脊束尾侧亚核(Sp5C)c-Fos免疫阳性细胞数。结果表明，夜间PRBS持续时间较长，SP5C内c-Fos免疫阳性细胞数明显高于白天。此外，提取小鼠三叉神经节(TG)，用实时定量聚合酶链式反应(RT-PCR)检测伤害性受体在白天和夜间的表达。结果表明，三叉神经节的瞬时受体潜伏期锚蛋白1mRNA的表达在夜间明显高于白天。这些结果表明，当啮齿动物活跃时，三叉神经区的疼痛在夜间比白天更强烈，部分原因是伤害性感受器表达的差异。
Management of time and circadian disruption is an extremely important factor in basic research on pain and analgesia. Although pain is known to vary throughout the day, the mechanism underlying this circadian variation remains largely unknown. In this study, we hypothesized that the process of pain transmission to the central nervous system (after receiving nociceptive stimuli from outside the body) would show day-night differences. Ten-week-old male mice were kept under a strict 12/12-h light/dark cycle for at least 10 days. Formalin was then injected into the second branch region of the trigeminal nerve and the duration of pain-related behaviors (PRBs) was assessed. Immunohistochemical staining was then performed, and the c-Fos-immunopositive cells in the trigeminal spinal tract subnucleus caudalis (Sp5C) were counted. The results showed that the duration of PRBs was longer and the number of c-Fos immunopositive cells in the Sp5C was higher at nighttime than during the day. In addition, the trigeminal ganglia (TG) were extracted from the mice and examined by quantitative real-time PCR to evaluate the daytime and nighttime expression of nociceptive receptors. The results showed that the mRNA expression of transient receptor potential ankyrin 1 in the TG was significantly higher at night than during the day. These results suggest that pain in the trigeminal nerve region is more intense at nighttime, when rodents are active, than during the daytime, partly due to differences in nociceptor expression.
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影响因子：
16.2
作者：
Baliki, Marwan N.;Geha, Paul Y.;Fields, Howard L.;Apkarian, A. Vania
通讯作者：
Apkarian, A. Vania
DOI：
10.1016/s0306-3623(98)00076-7
发表时间：
1998-11-01
期刊：
GENERAL PHARMACOLOGY
影响因子：
--
作者：
Konecka, AM;Sroczynska, I
通讯作者：
Sroczynska, I
影响因子：
7.4
作者：
Christianson CA;Corr M;Firestein GS;Mobargha A;Yaksh TL;Svensson CI
通讯作者：
Svensson CI
影响因子：
50.3
作者：
Kettner NM;Voicu H;Finegold MJ;Coarfa C;Sreekumar A;Putluri N;Katchy CA;Lee C;Moore DD;Fu L
通讯作者：
Fu L
影响因子：
4
作者：
Luccarini, Philippe;Childeric, Anne;Dallel, Radhouane
通讯作者：
Dallel, Radhouane