A robust electrical microcytometer with 3-dimensional hydrofocusing.
A robust electrical microcytometer with 3-dimensional hydrofocusing.
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DOI：
10.1039/b912214a
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发表时间：
2009-11-21
期刊：
影响因子：
6.1
通讯作者：
Bashir R
中科院分区：
文献类型：
--
作者：
Watkins N;Venkatesan BM;Toner M;Rodriguez W;Bashir R
在本文中，我们提出了一种装置，电计数血细胞群体使用交流阻抗询问技术在一个微型血细胞计数器（microcytometer）。具体而言，我们将注意力集中在获得人CD 4 + T淋巴细胞（辅助性T细胞）的浓度上，这是诊断艾滋病毒/艾滋病患者并对ARV（抗逆转录病毒）药物治疗的有效性进行准确预测的必要方法。我们通过流体模拟和相应的实验研究了一种简单制造的三维（3D）流体动力聚焦机制的有效性，以增加由粒子移位引起的阻抗脉冲的信噪比，并确保通过电感测区域的粒子移位高度的较低方差。我们发现，最佳的3D鞘流设置导致阻抗脉冲信噪比增加44.4%，此外还提供了更准确的粒度分布表示。我们的显微细胞计数器T细胞计数与使用行业标准流式细胞仪发现的T细胞计数非常接近，浓度范围超过三个数量级，样本体积约为一滴血（约20 μL）。此外，我们的设备显示出区分活的和死的/垂死的淋巴细胞群的能力。这种微量血细胞计数仪可以成为一种便携、快速、廉价的解决方案的基础，即使在世界上资源最贫乏的地区也可以获得活/死血细胞计数。
In this paper, we present a device to electrically count blood cell populations using an AC impedance interrogation technique in a microfabricated cytometer (microcytometer). Specifically, we direct our attention to obtaining the concentration of human CD4+ T lymphocytes (helper T cells), which is a necessary method to diagnose patients for HIV/AIDS and to give an accurate prognosis on the effectiveness of ARV (anti-retroviral) drug treatments. We study the effectiveness of a simple-to-fabricate 3-dimensional (3D) hydrodynamic focusing mechanism through fluidic simulations and corresponding experiments to increase the signal-to-noise ratio of impedance pulses caused by particle translocation and ensure lower variance in particle translocation height through the electrical sensing region. We found that the optimal 3D sheath flow settings result in a 44.4% increase in impedance pulse signal-to-noise ratio in addition to giving a more accurate representation of particle size distribution. Our microcytometer T cell counts closely with those found using an industry-standard flow cytometer for the concentration range of over three orders of magnitude and using a sample volume approximately the size of a drop of blood (~20 μL). In addition, our device displayed the capability to differentiate between live and dead/dying lymphocyte populations. This microcytometer can be the basis of a portable, rapid, inexpensive solution to obtaining live/dead blood cell counts even in the most resource-poor regions of the world.
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影响因子：
2.7
作者：
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通讯作者：
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作者：
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通讯作者：
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