Exercise-induced increase in M2 macrophages accelerates wound healing in young mice.
Exercise-induced increase in M2 macrophages accelerates wound healing in young mice.
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DOI：
10.14814/phy2.15447
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发表时间：
2022-10
影响因子：
2.5
通讯作者：
中科院分区：
文献类型：
--
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据报道，在伤口愈合期间进行中等强度的运动可以减少炎性细胞因子和趋化因子，加速伤口愈合。然而，其对巨噬细胞表型的影响以及运动加速伤口愈合的机制仍不清楚。本研究旨在探讨运动对创伤愈合过程中巨噬细胞表型的影响，并阐明血管生成与创伤愈合的关系。将12周龄雄性C57 BL/6 J小鼠分为久坐组（n = 6）和运动组（n = 6）。运动组进行中等强度跑台运动（9.0 m/min，60 min）10 d。使用F4/80+诱导型一氧化氮合酶（iNOS）+（M1巨噬细胞）、F4/80+转化生长因子-β（TGF-β）1+（M2巨噬细胞）和CD 31 + α平滑肌肌动蛋白（α-SMA）+（血管生成）进行双重免疫荧光分析。与久坐组相比，运动组的伤口愈合速度明显加快。从伤口愈合早期开始，运动显著抑制M1巨噬细胞浸润，增加M2巨噬细胞计数。运动也显著增加了血管生成。此外，运动组中M2巨噬细胞表型与血管生成显著相关，表明M2巨噬细胞和血管生成与加速伤口愈合有关。这些发现表明，中等强度的运动增加了M2巨噬细胞来源的TGF-β1，这可能与年轻小鼠血管生成和伤口愈合的增强有关。中等强度运动抑制了幼年小鼠M1巨噬细胞来源的iNOS，并增加了M2巨噬细胞来源的TGF-β1。此外，M2巨噬细胞的增加可能与伤口愈合和血管生成有关。
Moderate‐intensity exercise performed during wound healing has been reported to decrease inflammatory cytokines and chemokines and accelerate wound healing. However, its effect on macrophage phenotype and the mechanism by which exercise accelerates wound healing remain unclear. The purpose of this study was to investigate the effect of exercise on macrophage phenotype during wound healing and to clarify the relationship between angiogenesis and wound healing. 12‐week‐old male C57BL/6J mice were divided into sedentary (n = 6) and exercise groups (n = 6). The exercise group performed moderate‐intensity treadmill running exercise (9.0 m/min, 60 min) for 10 days. Double immunofluorescence analysis was performed using F4/80+ inducible nitric oxide synthase (iNOS)+ for M1 macrophages, F4/80+ transforming growth factor‐beta (TGF‐β)1+ for M2 macrophages, and CD31+ alpha smooth muscle actin (α‐SMA)+ for angiogenesis. The exercise group showed significantly accelerated wound healing compared with the sedentary group. From early wound healing onward, exercise significantly inhibited M1 macrophage infiltration and increased M2 macrophage count. Exercise also significantly increased angiogenesis. Furthermore, the M2 macrophage phenotype was significantly correlated with angiogenesis in the exercise group, indicating that M2 macrophages and angiogenesis are related to accelerated wound healing. These findings suggest that moderate‐intensity exercise increases TGF‐β1 derived from M2 macrophages, which may be associated with enhanced angiogenesis and wound healing in young mice. Moderate‐intensity exercise suppressed M1 macrophage‐derived iNOS and increased M2 macrophage‐derived TGF‐β1 in young mice. In addition, the increase in M2 macrophages may be associated with wound healing and angiogenesis.
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影响因子：
4
作者：
Krzyszczyk P;Schloss R;Palmer A;Berthiaume F
通讯作者：
Berthiaume F
DOI：
10.1097/01.mco.0000247476.02650.18
发表时间：
2006-11-01
影响因子：
3.1
作者：
Gleeson, Michael
通讯作者：
Gleeson, Michael
影响因子：
3.9
作者：
Baek, Kyung-Wan;Lee, Da-In;Kim, Ji-Seok
通讯作者：
Kim, Ji-Seok
影响因子：
20.1
作者：
Chawla A
通讯作者：
Chawla A
影响因子：
4.9
作者：
Frykberg RG;Banks J
通讯作者：
Banks J