Human chorionic gonadotrophin indirectly activates peripheral γδT cells to produce interleukin-10 during early pregnancy.
Human chorionic gonadotrophin indirectly activates peripheral γδT cells to produce interleukin-10 during early pregnancy.
复制标题
DOI：
10.1002/iid3.1119
复制
发表时间：
2024-01
影响因子：
3.2
通讯作者：
Li, Hong
中科院分区：
文献类型：
--
作者：
Li, Liman;Liu, Yuan;Zhou, Wenjie;Yang, Chuan;Feng, Ting;Li, Hong
人绒毛膜促性腺激素（hCG）的免疫调节特性已被确定为成功妊娠的关键。然而，hCG对妊娠早期外周血γδT细胞的影响尚未见报道。我们将纯化的γδT细胞和外周血单个核细胞（PBMC）与早孕相关浓度的hCG共培养，并通过流式细胞术检测γδT细胞免疫功能特性的变化。与非妊娠女性相比，早期妊娠女性中CD 69+和IL-10+ γδT细胞的比例增加。γδT细胞表达低水平的甘露糖受体（CD 206），而不是经典的hCG/LH受体。用早孕相关浓度的hCG直接处理纯化的γδT细胞可能对其免疫功能没有显著影响。有趣的是，当PBMC用相同宽范围的hCG浓度处理时，CD 69+和IL-10+ γδT细胞与总γδT细胞的比率显著增加。某些早孕相关的hCG浓度可以提高外周血CD 69+和IL-10+ γδT细胞的比例，有助于γδT细胞的活化和早孕期间的免疫耐受。然而，这些影响可能不是由直接的配体-受体相互作用强烈介导的，它们可能高度依赖于免疫微环境。我们的新观察提出了一个关于胎儿和母体免疫细胞之间存在的内分泌免疫对话的观点。外周血γδT细胞有助于在妊娠早期建立免疫耐受。由于γδT细胞甘露糖受体的低表达，血循环中hCG浓度的升高可能不直接影响γδT细胞的功能。然而，当PBMC与hCG孵育时，某些妊娠相关的hCG浓度可以增加Ki 67 +γδT细胞，CD 69 +γδT细胞和IL-10+γδT细胞的比例，这可能依赖于免疫微环境调节。
The immunomodulatory properties of human chorionic gonadotrophin (hCG) have been identified to be critical for successful pregnancy. However, the effects of hCG on peripheral γδT cells during early pregnancy have not been reported previously. We cocultured the purified γδT cells and peripheral blood mononuclear cells (PBMCs) with early pregnancy‐relevant hCG concentrations and investigated the changes in the immune functional characteristics of γδT cells via flow cytometry assays. The ratios of CD69+ and IL‐10+ γδT cells were increased in early pregnant women compared to nonpregnant women. γδT cells expressed low levels of the mannose receptor (CD206) instead of the classical hCG/LH receptor for hCG. The direct treatment of purified γδT cells with early pregnancy‐relevant hCG concentrations may have no significant effects on their immune functions. Interestingly, when PBMCs were treated with the same broad range of hCG concentrations, the ratios of CD69+ and IL‐10+ γδT cells to total γδT cells were significantly increased. Certain early pregnancy‐relevant hCG concentrations could enhance the ratios of peripheral CD69+ and IL‐10+ γδT cells, contributing to the activation of γδT cells and immunological tolerance during early pregnancy. However, these affects may not be strongly mediated by direct ligand–receptor interactions and they may highly depend on immune microenvironment. Our novel observations propose a perspective into the endocrine‐immune dialog that exists between the fetus and maternal immune cells. Peripheral γδT cells contribute to establishing immune tolerance in early pregnancy. Increased hCG concentrations in the blood circulation may not directly affected γδT cells functions because of the low expression of mannose receptor in γδT cells. However, when PBMCs incubated with hCG, certain pregnancy‐relevant hCG concentrations can increase the ratios of Ki67+γδT cells, CD69+γδT cells, and IL‐10+γδT cells, which might be dependent on the immune microenvironment modulation.
登录
查看更多内容
影响因子：
7.3
作者：
Qin, Dengke;Xu, Huihui;Chen, Zechuan;Deng, Xujing;Jiang, Shan;Zhang, Xiaoming;Bao, Shihua
通讯作者：
Bao, Shihua
影响因子：
8
作者：
Ma L;Papadopoulou M;Taton M;Genco F;Marchant A;Meroni V;Vermijlen D
通讯作者：
Vermijlen D
影响因子：
3.4
作者：
Cai, Dongli;Tang, Yunhui;Yao, Xiaoying
通讯作者：
Yao, Xiaoying
影响因子：
3.7
作者：
Barros-Martins, Joana;Bruni, Elena;Prinz, Immo
通讯作者：
Prinz, Immo
影响因子：
22.7
作者：
Lerner, Emily C. C.;Woroniecka, Karolina I. I.;D'Anniballe, Vincent M. M.;Wilkinson, Daniel S. S.;Mohan, Aditya A. A.;Lorrey, Selena J. J.;Waibl-Polania, Jessica;Wachsmuth, Lucas P. P.;Miggelbrink, Alexandra M. M.;Jackson, Joshua D. D.;Cui, Xiuyu;Raj, Jude A. A.;Tomaszewski, William H. H.;Cook, Sarah L. L.;Sampson, John H. H.;Patel, Anoop P. P.;Khasraw, Mustafa;Gunn, Michael D. D.;Fecci, Peter E. E.
通讯作者：
Fecci, Peter E. E.