Stimulation of TRPV1 by Green Laser Light.
Stimulation of TRPV1 by Green Laser Light.
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DOI：
10.1155/2012/857123
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Schwarz W
中科院分区：
文献类型：
--
作者：
Gu Q;Wang L;Huang F;Schwarz W
可见光的低强度激光照射作为一种医学治疗方法早在40多年前就已被引入，但其医学应用仍然存在争议。还介绍了穴位的激光刺激，并提出了肥大细胞脱颗粒的建议。 TRPV离子通道的激活可能参与脱粒。在这里，我们研究了TRPV 1是否可以作为激光诱导的肥大细胞激活的候选人。辣椒素激活TRPV 1导致脱粒。为了研究激光照射对TRPV 1的影响，我们使用非洲爪蟾卵母细胞作为表达和模型系统。我们发现TRPV 1可以通过以下方式在卵母细胞中功能性表达：（a）辣椒素（K 1/2 = 1.1 μM）激活，（B）温度超过42°C激活，（c）pH降低（从7.4到6.2）激活，（d）钌红抑制。红光（637 nm）和蓝光（406 nm）既不影响卵母细胞的膜电流，也不调节辣椒素诱导的电流。 相反，绿色激光（532 nm）产生TRPV 1的功率依赖性激活。总之，我们可以证明绿色光在细胞水平上有效激活TRPV 1。绿色光在何种程度上具有医学相关性需要进一步研究。
Low-level laser irradiation of visible light had been introduced as a medical treatment already more than 40 years ago, but its medical application still remains controversial. Laser stimulation of acupuncture points has also been introduced, and mast-cells degranulation has been suggested. Activation of TRPV ion channels may be involved in the degranulation. Here, we investigated whether TRPV1 could serve as candidate for laser-induced mast cell activation. Activation of TRPV1 by capsaicin resulted in degranulation. To investigate the effect of laser irradiation on TRPV1, we used the Xenopus oocyte as expression and model system. We show that TRPV1 can functionally be expressed in the oocyte by (a) activation by capsaicin (K 1/2 = 1.1 μM), (b) activation by temperatures exceeding 42°C, (c) activation by reduced pH (from 7.4 to 6.2), and (d) inhibition by ruthenium red. Red (637 nm) as well as blue (406 nm) light neither affected membrane currents in oocytes nor did it modulate capsaicin-induced current. In contrast, green laser light (532 nm) produced power-dependent activation of TRPV1. In conclusion, we could show that green light is effective at the cellular level to activate TRPV1. To which extend green light is of medical relevance needs further investigation.
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