A DArT marker genetic map of perennial ryegrass (Lolium perenne L.) integrated with detailed comparative mapping information; comparison with existing DArT marker genetic maps of Lolium perenne, L. multiflorum and Festuca pratensis.
A DArT marker genetic map of perennial ryegrass (Lolium perenne L.) integrated with detailed comparative mapping information; comparison with existing DArT marker genetic maps of Lolium perenne, L. multiflorum and Festuca pratensis.
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DOI：
10.1186/1471-2164-14-437
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发表时间：
2013-07-03
期刊：
影响因子：
4.4
通讯作者：
Armstead I
中科院分区：
文献类型：
--
作者：
King J;Thomas A;James C;King I;Armstead I
黑麦草和羊茅属（黑麦草属和羊茅属）是一种牧草和草皮草，广泛用于农业和休闲场所。它们被归为禾本科亚科，因此与小麦、大麦、黑麦和燕麦密切相关。近年来，开发了一种DArT阵列，可用于黑麦草和羊茅的标记和定位信息的生成。这为黑麦草和羊茅研究人员提供了一个潜在的共同标记集，可以将其与禾本科植物的比较基因组信息联系起来。建立了由7个连锁群组成的F2多年生黑麦草遗传图谱，共有1316个标记，总图谱长度为683 cM。标记集包括866个DArT标记和315个基于基因序列的标记。与以往多年生黑麦草和意大利黑麦草（L. multiflorum）的DArT标记比较，鉴定出共有的DArT标记有87个和105个，分别有94%和87%的标记指向同源连锁群。与草甸羊茅（F. pratensis）类似的比较发现，共有28个DArT标记，其中约50%映射到非同源连锁群。在二年生油菜中，DArT标记所跨越的遗传距离涵盖了与水稻、短茅和高粱的比较基因组关系所能描述的大部分区域。DArT标记可能是一种有用的黑麦草和羊茅的共同标记资源，尽管通过共同标记的定位来对不同种群进行比对的成功将受到种群相互关联程度的影响。在这项研究中，DArT和基于基因的标记的详细作图可能会在未来仅使用DArT标记的作图群体中推导出比较关系。
Ryegrasses and fescues (genera, Lolium and Festuca) are species of forage and turf grasses which are used widely in agricultural and amenity situations. They are classified within the sub-family Pooideae and so are closely related to Brachypodium distachyon, wheat, barley, rye and oats. Recently, a DArT array has been developed which can be used in generating marker and mapping information for ryegrasses and fescues. This represents a potential common marker set for ryegrass and fescue researchers which can be linked through to comparative genomic information for the grasses. A F2 perennial ryegrass genetic map was developed consisting of 7 linkage groups defined by 1316 markers and deriving a total map length of 683 cM. The marker set included 866 DArT and 315 gene sequence-based markers. Comparison with previous DArT mapping studies in perennial and Italian ryegrass (L. multiflorum) identified 87 and 105 DArT markers in common, respectively, of which 94% and 87% mapped to homoeologous linkage groups. A similar comparison with meadow fescue (F. pratensis) identified only 28 DArT markers in common, of which c. 50% mapped to non-homoelogous linkage groups. In L. perenne, the genetic distance spanned by the DArT markers encompassed the majority of the regions that could be described in terms of comparative genomic relationships with rice, Brachypodium distachyon, and Sorghum bicolor. DArT markers are likely to be a useful common marker resource for ryegrasses and fescues, though the success in aligning different populations through the mapping of common markers will be influenced by degrees of population interrelatedness. The detailed mapping of DArT and gene-based markers in this study potentially allows comparative relationships to be derived in future mapping populations characterised using solely DArT markers.
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影响因子：
2.7
作者：
Dumsday, JL;Smith, KF;Jones, ES
通讯作者：
Jones, ES
影响因子：
3.3
作者：
King, Julie;Armstead, Ian P.;King, Ian P.
通讯作者：
King, Ian P.
影响因子：
64.8
作者：
Vogel, John P.;Garvin, David F.;Baxter, Ivan
通讯作者：
Baxter, Ivan
影响因子：
2
作者：
Tomaszewski, Celine;Byrne, Stephen L.;Barth, Susanne
通讯作者：
Barth, Susanne
影响因子：
14.9
作者：
Ouyang S;Zhu W;Hamilton J;Lin H;Campbell M;Childs K;Thibaud-Nissen F;Malek RL;Lee Y;Zheng L;Orvis J;Haas B;Wortman J;Buell CR
通讯作者：
Buell CR