A New Vaccination Method Based on Phage NgoΦ6 and Its Phagemid Derivatives.
A New Vaccination Method Based on Phage NgoΦ6 and Its Phagemid Derivatives.
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DOI：
10.3389/fmicb.2022.793205
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发表时间：
2022
影响因子：
5.2
通讯作者：
Stein, Daniel C.
中科院分区：
文献类型：
--
作者：
Piekarowicz, Andrzej;Klyz, Aneta;Stein, Daniel C.
关键词：
基于淋病奈瑟氏菌丝状噬菌体NgoΦ6的噬菌粒颗粒用作疫苗递送系统。我们证明，纳入/与这些颗粒相关的宿主蛋白质可以由宿主细菌的染色体基因编码，或者来自能够在噬菌粒宿主中作为自主实体复制的质粒。从三种类型的细胞制备噬菌粒颗粒，即含有噬菌粒基因组作为自主质粒的鼠伤寒沙门氏菌[pBSKS：：Φ 6 fm（ST）]、含有整合到染色体中的噬菌粒[pBSKS：：Φ 6 fm（Hin）]的流感嗜血杆菌Rd和S.肠道血清鼠伤寒沙门氏菌[pMPMT 6：：Φ 6 fm（ST）]，含有编码丙型肝炎病毒包膜糖蛋白E1的额外质粒pE 1 HCV。使用约200 μg纯化的噬菌体颗粒免疫兔。从这三种菌株制备的噬菌粒颗粒都引发了大量的IgG抗体，其能够识别细菌宿主细胞和蛋白质，如通过ELISA和FACS分析所确定的。特异性抗S.肠道血清鼠伤寒沙门氏菌，抗H.抗E_1抗体效价为170 μg/ml（沙门氏菌）、80 μg/ml（H.抗E1 HCV为65 μg/ml。总的来说，这些数据表明，经典的噬菌体展示方法低估了丝状噬菌体作为一种新型免疫原递送系统的潜力。
Phagemid particles based on the Neisseria gonorrhoeae filamentous phage NgoΦ6 were used as a vaccine delivery system. We demonstrate that the host proteins incorporated into/associated with these particles can be encoded by chromosomal genes of the host bacterium or from plasmids able to replicate as an autonomous entity in the phagemid host. Phagemid particles were prepared from three types of cells, namely, Salmonella enterica ser. Typhimurium [pBSKS::Φ6fm(ST)] containing phagemid genome as an autonomous plasmid, Haemophilus influenzae Rd containing phagemid [pBSKS::Φ6fm(Hin)] integrated into the chromosome, and S. enterica ser. Typhimurium [pMPMT6::Φ6fm(ST)] containing an additional plasmid, pE1 HCV, encoding the Hepatitis C virus envelope glycoprotein E1. Approximately 200 μg of purified phage particles was used to immunize rabbits. The phagemid particles prepared from these three strains all elicited a large amount of IgG antibodies that were able to recognize bacterial host cells and proteins, as determined by ELISA and FACS analysis. The amount of specific anti-S. enterica ser. Typhimurium, anti-H. influenzae, and anti-E1 HCV antibodies elicited by vaccination was 170 μg/ml for anti-Salmonella, 80 μg/ml for anti-H. influenzae, and 65 μg/ml for anti-E1 HCV. Taken in toto, these data suggest that classical phage display methods have underestimated the potential for filamentous phage as a novel immunogen delivery system.
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影响因子：
11
作者：
Wu CH;Liu IJ;Lu RM;Wu HC
通讯作者：
Wu HC
影响因子：
5.2
作者：
Henry KA;Arbabi-Ghahroudi M;Scott JK
通讯作者：
Scott JK
DOI：
10.1073/pnas.96.11.6025
发表时间：
1999-05-25
影响因子：
11.1
作者：
Gao, CS;Mao, SL;Janda, KD
通讯作者：
Janda, KD
DOI：
10.1093/bfgp/1.2.189
发表时间：
2002-07-01
期刊：
Briefings in Functional Genomics & Proteomics
影响因子：
--
作者：
Carmen, Sara;Jermutus, Lutz
通讯作者：
Jermutus, Lutz
影响因子：
5.7
作者：
Roth KDR;Wenzel EV;Ruschig M;Steinke S;Langreder N;Heine PA;Schneider KT;Ballmann R;Fühner V;Kuhn P;Schirrmann T;Frenzel A;Dübel S;Schubert M;Moreira GMSG;Bertoglio F;Russo G;Hust M
通讯作者：
Hust M