BrUFO positively regulates the infection of Chinese cabbage by Plasmodiophora brassicae.
BrUFO positively regulates the infection of Chinese cabbage by Plasmodiophora brassicae.
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DOI：
10.3389/fpls.2023.1128515
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发表时间：
2023
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
大白菜是我国最重要的蔬菜作物之一。但由根肿病菌侵染引起的根肿病严重影响了大白菜的产量和品质。在前期的研究中，我们发现BrUFO基因在接种芸苔疫霉后的大白菜病根中表达显著上调。在泛素介导的蛋白质水解过程中，UFO（不寻常的花器官）具有底物识别的特性。多种植物都可以通过泛素化途径激活免疫应答。因此，研究UFO在油菜疫霉侵染过程中的作用具有重要意义。本研究采用qRT-PCR和原位杂交技术检测BrUFO基因的表达谱。亚细胞定位法确定BrUFO在细胞内的表达位置。病毒诱导的基因沉默（VIGS）验证了BrUFO的功能。利用酵母双杂交技术筛选与BrUFO蛋白相互作用的蛋白。实时荧光定量PCR和原位杂交分析表明，BrUFO基因在抗性植株中的表达量低于感病植株。亚细胞定位分析表明BrUFO基因在细胞核中表达。病毒诱导的基因沉默（VIGS）分析表明，BrUFO基因的沉默降低了根肿病的发病率。用Y2 H法筛选出6个与BrUFO蛋白相互作用的蛋白。其中两个（Bra 038955，B细胞受体相关的31样蛋白和Bra 021273，GDSL基序酯酶/酰基转移酶/脂肪酶）被证实与BrUFO蛋白强烈相互作用。 BrUFO基因可能是大白菜抗疫霉侵染的关键基因。BrUFO基因沉默提高植物对根肿病的抗性。BrUFO蛋白可能通过GDSL脂肪酶与RP 2相互作用，诱导PRR介导的PTI反应中的泛素化，从而达到大白菜抗芸苔疫霉侵染的效果。
Chinese cabbage is one of the most important vegetable crops in China. However, the clubroot disease caused by the infection of Plasmodiophora brassicae (P. brassicae) has seriously affected the yield and quality of Chinese cabbage. In our previous study, BrUFO gene was found to be significantly up-regulated in diseased roots of Chinese cabbage after inoculation with P. brassicae. UFO (UNUSUAL FLORAL ORGANS) have the properties of substrate recognition during ubiquitin-mediated proteolysis. A variety of plant can activate immunity response through the ubiquitination pathway. Therefore, it is very important to study the function of UFO in response to P. brassicae. In this study, The expression pattern of BrUFO Gene was measured by qRT-PCR and In situ Hybridization (ISH). The expression location of BrUFO in cells was determined by subcellular localization. The function of BrUFO was verified by Virus-induced Gene Silencing (VIGS). proteins interacting with BrUFO protein were screened by yeast two-hybrid. Quantitative real-time polymerase chain reactions (qRT-PCR) and in situ hybridization analysis showed that expression of BrUFO gene in the resistant plants was lower than that in susceptible plants. Subcellular localization analysis showed that BrUFO gene was expressed in the nucleus. Virus-induced gene silencing (VIGS) analysis showed that silencing of BrUFO gene reduced the incidence of clubroot disease. Six proteins interacting with BrUFO protein were screened by Y2H assay. Two of them (Bra038955, a B-cell receptor-associated 31-like protein and Bra021273, a GDSL-motif esterase/acyltransferase/lipase Enzyme) were confirmed to strongly interact with BrUFO protein. BrUFO gene should be a key gene of chinese cabbage against the infection of P. brassicae. BrUFO gene silencing improves the resistance of plants to clubroot disease. BrUFO protein may interact with CUS2 to induce ubiquitination in PRR-mediated PTI reaction through GDSL lipases, so as to achieve the effect of Chinese cabbage against the infection of P. brassicae.
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