Macrophage polarization in response to ECM coated polypropylene mesh.
Macrophage polarization in response to ECM coated polypropylene mesh.
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DOI：
10.1016/j.biomaterials.2014.04.115
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发表时间：
2014-08
期刊：
影响因子：
14
通讯作者：
Badylak, Stephen F.
中科院分区：
文献类型：
--
作者：
Wolf, Matthew T.;Dearth, Christopher L.;Ranallo, Christian A.;LoPresti, Samuel T.;Carey, Lisa E.;Daly, Kerry A.;Brown, Bryan N.;Badylak, Stephen F.
宿主对植入生物材料的反应是一个高度调控的过程，影响设备的功能和临床结果。不可降解的生物材料，如针织聚丙烯网，经常引起慢性异物反应，导致纤维化。先前的研究表明，聚丙烯网的细胞外基质（ECM）水凝胶涂层降低了异物反应的强度，尽管其作用方式尚不清楚。巨噬细胞的参与在异体对生物材料反应的发展中起着关键作用，因此本研究研究了补片植入后巨噬细胞的极化。研究人员对未涂覆的聚丙烯网片和涂覆了来自真皮或膀胱的ECM水凝胶的水合和干燥形式的网片进行了巨噬细胞极化的时空分析。在3-35 d期间定量促炎M1巨噬细胞（CD86+/CD68+）、交替活化M2巨噬细胞（CD206+/CD68+）和异物巨细胞。未涂覆的聚丙烯网在网状纤维表面引起了显性的M1响应，从第7天开始，每一种ECM涂层类型都降低了M1响应。在第14天和第35天，M1反应的减弱伴随着异物巨细胞数量的减少，尽管在任何时候对M2巨噬细胞数量的影响都很小。这些结果表明，ECM涂层在体内减弱了M1巨噬细胞的反应，增加了M2/M1与聚丙烯网的比值。
The host response to implanted biomaterials is a highly regulated process that influences device functionality and clinical outcome. Non-degradable biomaterials, such as knitted polypropylene mesh, frequently elicit a chronic foreign body reaction with resultant fibrosis. Previous studies have shown that an extracellular matrix (ECM) hydrogel coating of polypropylene mesh reduces the intensity of the foreign body reaction, though the mode of action is unknown. Macrophage participation plays a key role in the development of the foreign body reaction to biomaterials, and therefore the present study investigated macrophage polarization following mesh implantation. Spatiotemporal analysis of macrophage polarization was conducted in response to uncoated polypropylene mesh and mesh coated with hydrated and dry forms of ECM hydrogels derived from either dermis or urinary bladder. Pro-inflammatory M1 macrophages (CD86+/CD68+), alternatively activated M2 macrophages (CD206+/CD68+), and foreign body giant cells were quantified between 3-35 days. Uncoated polypropylene mesh elicited a dominant M1 response at the mesh fiber surface, which was decreased by each ECM coating type beginning at 7 days. The diminished M1 response was accompanied by a reduction in the number of foreign body giant cells at 14 and 35 days, though there was a minimal effect upon the number of M2 macrophages at any time. These results show that ECM coatings attenuate the M1 macrophage response and increase the M2/M1 ratio to polypropylene mesh in vivo.
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影响因子：
12.3
作者：
Carpenter AE;Jones TR;Lamprecht MR;Clarke C;Kang IH;Friman O;Guertin DA;Chang JH;Lindquist RA;Moffat J;Golland P;Sabatini DM
通讯作者：
Sabatini DM
影响因子：
14
作者：
Freytes, Donald O.;Martin, Jeffrey;Badylak, Stephen F.
通讯作者：
Badylak, Stephen F.
影响因子：
14
作者：
Badylak, Stephen F.
通讯作者：
Badylak, Stephen F.
影响因子：
14
作者：
Brown, Bryan N.;Valentin, Jolene E.;Stewart-Akers, Ann M.;McCabe, George P.;Badylak, Stephen F.
通讯作者：
Badylak, Stephen F.
DOI：
10.1007/s00464-008-9936-6
发表时间：
2008-12-01
影响因子：
3.1
作者：
Hollinsky, Christian;Sandberg, Simone;Seidler, Sabine
通讯作者：
Seidler, Sabine