Effect of Stimulus Waveform of Biphasic Current Pulse on Retinal Ganglion Cell Responses in Retinal Degeneration (rd1) mice.
Effect of Stimulus Waveform of Biphasic Current Pulse on Retinal Ganglion Cell Responses in Retinal Degeneration (rd1) mice.
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DOI：
10.4196/kjpp.2015.19.2.167
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发表时间：
2015-03
期刊：
影响因子：
--
通讯作者：
Goo YS
中科院分区：
文献类型：
--
作者：
Ahn KN;Ahn JY;Kim JH;Cho K;Koo KI;Senok SS;Goo YS
一种视网膜假体正在开发中，用于恢复视网膜色素变性(RP)和老年性黄斑变性(AMD)患者的视力。确定假体的最佳电刺激参数是开发可行的视网膜假体的最重要的因素之一。在这里，我们研究了不同电荷平衡的双相脉冲在诱发视网膜神经节细胞(RGC)反应方面的效果。选用视网膜变性(Rd1)小鼠(n=17)。从体外视网膜制备开始，将视网膜贴片置于8×8多电极阵列(MEA)上，在施加电刺激时记录RGC反应。对于非对称脉冲，脉冲的第一相与对称脉冲相同，但第二相的幅度小于10µA，并且通过延长脉冲的持续时间来满足电荷平衡条件。对于强度(或持续时间)调制，脉冲的持续时间(或幅度)固定为500微米S(30微安)，强度(或持续时间)在2至60微安(60微安至1000微安)之间变化。当PSTH在刺激后400ms内出现多个(3~4)峰，且棘波数目至少比刺激前400ms时多30%时，视网膜节细胞为反应阳性。RGC响应被阳极和阴极第一相两相脉冲很好地调制。无论脉冲的对称性如何，阴极第一相脉冲对RGC活性的调制明显好于阳极第一相脉冲。
A retinal prosthesis is being developed for the restoration of vision in patients with retinitis pigmentosa (RP) and age-related macular degeneration (AMD). Determining optimal electrical stimulation parameters for the prosthesis is one of the most important elements for the development of a viable retinal prosthesis. Here, we investigated the effects of different charge-balanced biphasic pulses with regard to their effectiveness in evoking retinal ganglion cell (RGC) responses. Retinal degeneration (rd1) mice were used (n=17). From the ex-vivo retinal preparation, retinal patches were placed ganglion cell layer down onto an 8×8 multielectrode array (MEA) and RGC responses were recorded while applying electrical stimuli. For asymmetric pulses, 1st phase of the pulse is the same with symmetric pulse but the amplitude of 2nd phase of the pulse is less than 10 µA and charge balanced condition is satisfied by lengthening the duration of the pulse. For intensities (or duration) modulation, duration (or amplitude) of the pulse was fixed to 500 µs (30 µA), changing the intensities (or duration) from 2 to 60 µA (60 to 1000 µs). RGCs were classified as response-positive when PSTH showed multiple (3~4) peaks within 400 ms post stimulus and the number of spikes was at least 30% more than that for the immediate pre-stimulus 400 ms period. RGC responses were well modulated both with anodic and cathodic phase-1st biphasic pulses. Cathodic phase-1st pulses produced significantly better modulation of RGC activity than anodic phase-1st pulses regardless of symmetry of the pulse.
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影响因子：
3
作者：
Grumet, AE;Wyatt, JL;Rizzo, JF
通讯作者：
Rizzo, JF
DOI：
10.1016/0302-4598(92)80067-q
发表时间：
1992-12-01
期刊：
BIOELECTROCHEMISTRY AND BIOENERGETICS
影响因子：
--
作者：
JIMBO, Y;KAWANA, A
通讯作者：
KAWANA, A
DOI：
10.1109/tbcas.2008.918285
发表时间：
2008-03-01
影响因子：
5.1
作者：
Brown, Edgar A.;Ross, James D.;DeWeerth, Stephen P.
通讯作者：
DeWeerth, Stephen P.
影响因子：
56.9
作者：
LILLY, JC;HUGHES, JR;GALKIN, TW
通讯作者：
GALKIN, TW
影响因子：
2.5
作者：
Fried, Shelley I.;Lasker, Aaron C. W.;Rizzo, Joseph F., III
通讯作者：
Rizzo, Joseph F., III