Sex-dimorphic gene expression and ineffective dosage compensation of Z-linked genes in gastrulating chicken embryos.
Sex-dimorphic gene expression and ineffective dosage compensation of Z-linked genes in gastrulating chicken embryos.
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DOI：
10.1186/1471-2164-11-13
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发表时间：
2010-01-07
期刊：
影响因子：
4.4
通讯作者：
Pourquié O
中科院分区：
文献类型：
--
作者：
Zhang SO;Mathur S;Hattem G;Tassy O;Pourquié O
在模式生物如C. elegans、果蝇和M.肌肉引人注目的是，这三种模式生物的性别决定和剂量补偿机制有很大不同。鸟类还有另一种情况，即异配性别是雌性。鸟类的性别决定仍然知之甚少，到目前为止，很少有关键的决定因素被确定。与大多数其他物种相比，鸟类性染色体基因的剂量补偿似乎相当无效。通过比较来自单个鸡胚的显微切割的原始条纹的微阵列，我们在非常早期的阶段（Hamburger和汉密尔顿阶段4），在任何性分化发生之前，鉴定了大量在雄性和雌性胚胎之间差异表达的基因。这些基因大多位于Z染色体上，这表明剂量补偿在早期鸡胚中无效。基因本体分析，使用增强的注释工具，在我们的实验室（称为Manteia）开发的鸡基因组的Affyestival probesets，显示在这些男性偏向的基因中发现的Z染色体，超过20个基因在性别分化中发挥作用。这些结果证实了以前的研究表明，相当低效的剂量补偿的Z染色体的鸟类，并表明，这种性别的基因调控的二型性早在性分化的开始。这些数据也表明了非补偿性Z连锁基因在鸟类体细胞性别分化中的潜在作用。
Considerable progress has been made in our understanding of sex determination and dosage compensation mechanisms in model organisms such as C. elegans, Drosophila and M. musculus. Strikingly, the mechanism involved in sex determination and dosage compensation are very different among these three model organisms. Birds present yet another situation where the heterogametic sex is the female. Sex determination is still poorly understood in birds and few key determinants have so far been identified. In contrast to most other species, dosage compensation of bird sex chromosomal genes appears rather ineffective. By comparing microarrays from microdissected primitive streak from single chicken embryos, we identified a large number of genes differentially expressed between male and female embryos at a very early stage (Hamburger and Hamilton stage 4), long before any sexual differentiation occurs. Most of these genes are located on the Z chromosome, which indicates that dosage compensation is ineffective in early chicken embryos. Gene ontology analyses, using an enhanced annotation tool for Affymetrix probesets of the chicken genome developed in our laboratory (called Manteia), show that among these male-biased genes found on the Z chromosome, more than 20 genes play a role in sex differentiation. These results corroborate previous studies demonstrating the rather inefficient dosage compensation for Z chromosome in birds and show that this sexual dimorphism in gene regulation is observed long before the onset of sexual differentiation. These data also suggest a potential role of non-compensated Z-linked genes in somatic sex differentiation in birds.
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小鼠中的剂量补偿平衡了X连锁基因的上调和沉默。
影响因子：
9.8
作者：
Lin, Hong;Gupta, Vibhor;Vermilyea, Matthew D;Falciani, Francesco;Lee, Jeannie T;O'Neill, Laura P;Turner, Bryan M
通讯作者：
Turner, Bryan M
影响因子：
2.5
作者：
OBERKOTTER, LV;TENORE, A;KOLDOVSKY, O
通讯作者：
KOLDOVSKY, O
影响因子：
56.9
作者：
Dequeant, Mary-Lee;Glynn, Earl;Pourquie, Olivier
通讯作者：
Pourquie, Olivier
影响因子：
64.5
作者：
BURGOYNE, PS;MAHADEVAIAH, SK;PALMER, SJ
通讯作者：
PALMER, SJ
影响因子：
4.8
作者：
London, Sarah E.;Monks, D. Ashley;Schlinger, Barney A.
通讯作者：
Schlinger, Barney A.