Equivalent susceptibility of Anopheles gambiae M and S molecular forms and Anopheles arabiensis to Plasmodium falciparum infection in Burkina Faso.
Equivalent susceptibility of Anopheles gambiae M and S molecular forms and Anopheles arabiensis to Plasmodium falciparum infection in Burkina Faso.
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Anopheles gambiae M和S分子形式的等效敏感性以及阿拉伯菌对伯基纳法索（Burkina Faso）疟原虫感染的阿拉伯菌。
DOI：
10.1186/1475-2875-12-204
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发表时间：
2013-06-14
期刊：
影响因子：
3
通讯作者：
Vernick KD
中科院分区：
文献类型：
--
作者：
Gnémé A;Guelbéogo WM;Riehle MM;Sanou A;Traoré A;Zongo S;Eiglmeier K;Kabré GB;Sagnon N;Vernick KD
广义冈比亚按蚊（Anopheles gambiae sensu lato（s.l.）布基纳法索的按蚊种复合体由阿拉伯按蚊和冈比亚按蚊的分子形式M和S组成。之前比较M型和S型恶性疟原虫感染水平的研究得出了相互矛盾的结果。从天然池中采集苔藓虫幼虫样本，在受控条件下饲养至成年，并通过实验性喂食配子体载体的血液，用天然恶性疟原虫进行攻击。感染后一周测定卵囊感染率和感染强度。对尸体的DNA进行基因分型，以确定物种和分子形式。总共有7，400只从野外捕获的幼虫生长的成年蚊子在跨越四个传播季节的29次实验感染中受到配子体的挑战。总感染率平均为40.7%。gambiae M型，占41.4%。gambiae S型，占40.1%;阿拉伯的。三个人群之间的感染率或感染强度没有显著差异。值得注意的是，在相同感染性血液上平行挑战群体组的感染实验显示群体组之间的感染差异较小，而群体组组成不太平衡的感染具有较低的统计功效，并且显示出更经常从零平均值波动的明显差异。该研究清楚地表明，在布基纳法索的研究地点，在三个同域种群之间，对恶性疟原虫感染的遗传易感性没有差异。冈比亚公司复杂.用相同的感染性血粉喂养蚊子群体大大增加了统计功效。相反，比较感染之间而不是感染之内的不同蚊子群体会产生蚊子群体之间更大的明显差异，这是由于统计能力较低和噪音较大，并可能导致假阳性结果。在进行人群之间的感染比较时，比较不同人群同时摄入相同的感染性血液后的感染情况更为准确。
The Anopheles gambiae sensu lato (s.l.) species complex in Burkina Faso consists of Anopheles arabiensis, and molecular forms M and S of Anopheles gambiae sensu stricto (s.s.). Previous studies comparing the M and S forms for level of infection with Plasmodium falciparum have yielded conflicting results. Mosquito larvae were sampled from natural pools, reared to adulthood under controlled conditions, and challenged with natural P. falciparum by experimental feeding with blood from gametocyte carriers. Oocyst infection prevalence and intensity was determined one week after infection. DNA from carcasses was genotyped to identify species and molecular form. In total, 7,400 adult mosquitoes grown from wild-caught larvae were challenged with gametocytes in 29 experimental infections spanning four transmission seasons. The overall infection prevalence averaged 40.7% for A. gambiae M form, 41.4% for A. gambiae S form, and 40.1% for A. arabiensis. There was no significant difference in infection prevalence or intensity between the three population groups. Notably, infection experiments in which the population groups were challenged in parallel on the same infective blood displayed less infection difference between population groups, while infections with less balanced composition of population groups had lower statistical power and displayed apparent differences that fluctuated more often from the null average. The study clearly establishes that, at the study site in Burkina Faso, there is no difference in genetic susceptibility to P. falciparum infection between three sympatric population groups of the A. gambiae s.l. complex. Feeding the mosquito groups on the same infective blood meal greatly increases statistical power. Conversely, comparison of the different mosquito groups between, rather than within, infections yields larger apparent difference between mosquito groups, resulting from lower statistical power and greater noise, and could lead to false-positive results. In making infection comparisons between population groups, it is more accurate to compare the different groups after feeding simultaneously upon the same infective blood.
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影响因子：
6.7
作者：
Mitri C;Jacques JC;Thiery I;Riehle MM;Xu J;Bischoff E;Morlais I;Nsango SE;Vernick KD;Bourgouin C
通讯作者：
Bourgouin C
影响因子：
3.7
作者：
Boissière A;Gimonneau G;Tchioffo MT;Abate L;Bayibeki A;Awono-Ambéné PH;Nsango SE;Morlais I
通讯作者：
Morlais I
影响因子：
3
作者：
Ndiath MO;Cohuet A;Gaye A;Konate L;Mazenot C;Faye O;Boudin C;Sokhna C;Trape JF
通讯作者：
Trape JF
影响因子：
3.8
作者：
della Torre, A;Tu, ZJ;Petrarca, V
通讯作者：
Petrarca, V
影响因子：
56.9
作者：
Niaré, O;Markianos, K;Vernick, KD
通讯作者：
Vernick, KD