CRISPR/Cas9 suppression of OsAT10, a rice BAHD acyltransferase, reduces p-coumaric acid incorporation into arabinoxylan without increasing saccharification.
CRISPR/Cas9 suppression of OsAT10, a rice BAHD acyltransferase, reduces p-coumaric acid incorporation into arabinoxylan without increasing saccharification.
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DOI：
10.3389/fpls.2022.926300
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
酯连接的羟基肉桂酸(FA)和对香豆酸(p-CA)在细胞壁阿拉伯木聚糖(AX)和草细胞壁中AX与木质素之间的交联中起着重要的作用。羟基肉桂酸酯加成到AX上，是由BAHD酰基辅酶A利用转移酶的Mitchell分支介导的。OsAT10(一种Mitchell分支BAHD酰基转移酶)在水稻中的过表达，先前已被证明增加了叶和茎中AX中的p-CA含量，导致细胞壁消化率增加，可能与FA含量的降低有关。为了研究OsAT10的生理作用，我们建立了缺失OsAT10的水稻CRISPR/Cas9基因敲除突变体。我们对野生型植物中羟基肉桂酸含量的分析表明，与AX相关的p-CA几乎只在稻壳中发现，在其他组织中很少发现。突变植株基本上不存在与AX相关的酯连接的p-CA，这表明OsAT10是水稻植株中负责将p-CA加到阿拉伯木聚糖上的主要酶。我们没有发现缺乏与AX相关的p-CA的稻壳的消化率变化，这表明在OsAT10过度表达的植株中所看到的消化率的变化完全是由于与AX相关的FA的补偿性降低。
Ester-linked hydroxycinnamic acids ferulic acid (FA) and para-coumaric acid (p-CA) play important roles in crosslinking within cell wall arabinoxylans (AX) and between AX and lignin in grass cell walls. The addition of hydroxycinnamates to AX, is mediated by the Mitchell clade of BAHD acyl-coenzyme A-utilizing transferases. Overexpression of OsAT10 (a Mitchell clade BAHD acyl transferase) in rice, has previously been shown to increase p-CA content in AX in leaves and stems, leading to increased cell wall digestibility, potentially associated with a concomitant decrease in FA content. To investigate the physiological role of OsAT10 we established CRISPR/Cas9 rice knock-out mutants devoid of OsAT10. Our analysis of hydroxycinnamic acid content in wild type plants revealed that AX associated p-CA is found almost exclusively in rice husks, with very little found in other tissues. Mutant plants were essentially devoid of ester-linked p-CA associated with AX, indicating that OsAT10 represents the major enzyme responsible for the addition of p-CA to arabinoxylan in rice plants. We found no change in the digestibility of rice husk lacking AX-associated p-CA, suggesting that the changes in digestibility seen in OsAT10 overexpressing plants were solely due to compensatory decreases in AX-associated FA.
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DOI：
10.1111/nph.14970
发表时间：
2018-04
期刊：
The New phytologist
影响因子：
--
作者：
de Souza WR;Martins PK;Freeman J;Pellny TK;Michaelson LV;Sampaio BL;Vinecky F;Ribeiro AP;da Cunha BADB;Kobayashi AK;de Oliveira PA;Campanha RB;Pacheco TF;Martarello DCI;Marchiosi R;Ferrarese-Filho O;Dos Santos WD;Tramontina R;Squina FM;Centeno DC;Gaspar M;Braga MR;Tiné MAS;Ralph J;Mitchell RAC;Molinari HBC
通讯作者：
Molinari HBC
影响因子：
5.2
作者：
Goto, M;Ehara, H;Morita, O
通讯作者：
Morita, O
影响因子：
4.7
作者：
Chari R;Yeo NC;Chavez A;Church GM
通讯作者：
Church GM
影响因子：
3.6
作者：
Kim, Hoon;Ralph, John;Akiyama, Takuya
通讯作者：
Akiyama, Takuya
DOI：
10.1073/pnas.1202079109
发表时间：
2012-10-16
影响因子：
11.1
作者：
Chiniquy, Dawn;Sharma, Vaishali;Ronald, Pamela C.
通讯作者：
Ronald, Pamela C.