Planthopper-Secreted Salivary Calmodulin Acts as an Effector for Defense Responses in Rice.
Planthopper-Secreted Salivary Calmodulin Acts as an Effector for Defense Responses in Rice.
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DOI：
10.3389/fpls.2022.841378
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发表时间：
2022
影响因子：
5.6
通讯作者：
Ji R
中科院分区：
文献类型：
--
作者：
Fu J;Shi Y;Wang L;Tian T;Li J;Gong L;Zheng Z;Jing M;Fang J;Ji R
褐飞虱（Nilaparvata lugens，BPH）和灰飞虱（Laodelphax striatellus，SBPH）是亚洲水稻的主要害虫。这些刺吸式昆虫在进食过程中向宿主分泌唾液。尽管如此，稻飞虱如何利用唾液效应器持续取食水稻仍是一个未知数。在这里，我们筛选了他们的唾液蛋白质组，并选择了8个唾液蛋白之间的SBPH和BPH的候选效应。沉默钙调素（CaM）可阻止褐飞虱和褐飞虱穿透韧皮部。因此，它们的食物摄入量，存活率和在水稻植株上的繁殖力降低。相比之下，CaM沉默对人工饲料饲养的BPH和SBPH的存活率影响较小。BPH和SBPH的CaM氨基酸序列相同。CaM在唾液腺中高表达，并在取食过程中分泌到水稻植株中。细菌表达的重组CaM蛋白具有钙结合活性。在植物中表达表明，CaM定位于植物细胞质和细胞核，并抑制植物防御，如过氧化氢（H2O2）的积累和胼胝质沉积。CaM沉默的BPH和SBPH在水稻植株中引起相对高水平的H2O2和胼胝质积累。上述结果表明，CaM是一种效应子，因为它通过抑制水稻中胼胝质的沉积和H2O2的积累而使稻飞虱能够到达韧皮部。
The brown planthopper (Nilaparvata lugens, BPH) and small brown planthopper (Laodelphax striatellus, SBPH) are major pests of rice (Oryza sativa) in Asia. These piercing-sucking insects secrete saliva into the host during feeding. Nevertheless, it is largely unknown how planthoppers use salivary effectors to enable continuous feeding on rice. Here, we screened their salivary proteomes and selected eight salivary proteins conserved between SBPH and BPH as candidate effectors. Silencing calmodulin (CaM) impeded BPH and SBPH from penetrating the phloem. Hence, their food intake, survival, and fecundity on rice plants were reduced. By contrast, CaM silencing had a small effect on the survival rate of BPH and SBPH raised on artificial diet. The CaM amino acid sequences were the same for both BPH and SBPH. CaM was highly expressed in their salivary glands and secreted into the rice plants during feeding. Bacterially expressed recombinant CaM protein exhibited calcium-binding activity. In planta expression disclosed that CaM was localized to the plant cytoplasms and nuclei and suppressed plant defenses such as hydrogen peroxide (H2O2) accumulation and callose deposition. CaM-silenced BPH and SBPH nymphs elicited relatively high levels of H2O2 and callose accumulation in rice plants. The foregoing results reveal that CaM is an effector as it enables the planthopper to reach the phloem by suppressing callose deposition and H2O2 accumulation in rice.
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影响因子：
7.4
作者：
Hao, Peiying;Liu, Caixiang;He, Guangcun
通讯作者：
He, Guangcun
影响因子：
6.9
作者：
Bolwell, GP;Bindschedler, LV;Minibayeva, F
通讯作者：
Minibayeva, F
DOI：
10.1073/pnas.1407687111
发表时间：
2014-06-17
影响因子：
11.1
作者：
Chaudhary, Ritu;Atamian, Hagop S.;Kaloshian, Isgouhi
通讯作者：
Kaloshian, Isgouhi
DOI：
10.1094/mpmi-01-14-0018-r
发表时间：
2014-07
期刊：
Molecular plant-microbe interactions : MPMI
影响因子：
--
作者：
Elzinga DA;De Vos M;Jander G
通讯作者：
Jander G
通过转录组学和 LC-MS/MS 分析鉴定烟粉虱唾液蛋白
影响因子：
4
作者：
Huang, Hai-Jian;Ye, Zhuang-Xin;Li, Jun-Min
通讯作者：
Li, Jun-Min