Sex and estrogen receptor expression influence opioid peptide levels in the mouse hippocampal mossy fiber pathway.
Sex and estrogen receptor expression influence opioid peptide levels in the mouse hippocampal mossy fiber pathway.
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DOI：
10.1016/j.neulet.2013.07.048
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发表时间：
2013-09-27
影响因子：
2.5
通讯作者：
Milner TA
中科院分区：
文献类型：
--
作者：
Van Kempen TA;Kahlid S;Gonzalez AD;Spencer-Segal JL;Tsuda MC;Ogawa S;McEwen BS;Waters EM;Milner TA
阿片肽强啡肽(Dyn)和脑啡肽(L-enk)存在于海马苔藓纤维通路中，在那里它们调节突触的可塑性。在大鼠中，当雌激素水平升高时，Dyn和L-ENK免疫反应(-ir)的水平增加(Torres-Reveron等人。2008年和2009年)。在这里，我们使用定量免疫细胞化学来检查阿片类药物水平在野生型(WT)小鼠的发情周期中是否受到类似的调节，以及这些与雄性小鼠相比如何。此外，使用雌激素受体(ER)α和β基因敲除小鼠(分别为AERKO和BERKO)，本研究考察了雌激素受体在快速、膜启动(6小时)或较慢的核启动(48小时)雌二醇对苔藓纤维阿片水平影响中的作用。与大鼠不同，Dyn和L-ENK-ir的水平在发情周期中没有变化。但与男性相比，女性CA3a的Dyn-ir和CA3b的L-enk-ir水平较高。在WT和BERKO去卵巢(OVX)小鼠，给予苯甲酸雌二醇组(EB)6小时和48小时后，Dyn-和L-ENK-ir均无明显变化。然而，仅AERKO小鼠齿状回(DG)和CA3b区Dyn-ir在EB后48h显著升高。这些发现表明，在小鼠苔藓纤维通路中，循环激素水平不像在大鼠那样调节Dyn或L-ENK的水平。这可能是由于苔藓纤维途径的物种差异所致。然而，在小鼠中，在没有ERα的情况下，Dyn水平受到外源EB的调节，可能是通过ERβ介导的需要新基因转录的途径。
The opioid peptides, dynorphin (DYN) and enkephalin (L-ENK) are contained in the hippocampal mossy fiber pathway where they modulate synaptic plasticity. In rats, the levels of DYN and L-ENK immunoreactivity (-ir) are increased when estrogen levels are elevated (Torres-Reveron et al. 2008 and 2009). Here, we used quantitative immunocytochemistry to examine whether opioid levels are similarly regulated in wildtype (WT) mice over the estrous cycle, and how these compared to males. Moreover, using estrogen receptor (ER) alpha and beta knockout mice (AERKO and BERKO, respectively), the present study examined the role of ERs in rapid, membrane-initiated (6 hr), or slower, nucleus-initiated (48 hr) estradiol effects on mossy fiber opioid levels. Unlike rats, the levels of DYN and L-ENK-ir did not change over the estrous cycle. However, compared to males, females had higher levels of DYN-ir in CA3a and L-ENK-ir in CA3b. In WT and BERKO ovariectomized (OVX) mice, neither DYN- nor L-ENK-ir changed following 6 or 48 hrs estradiol benzoate (EB) administration. However, DYN-ir significantly increased 48 hours after EB in the dentate gyrus (DG) and CA3b of AERKO mice only. These findings suggest that cyclic hormone levels regulate neither DYN nor L-ENK levels in the mouse mossy fiber pathway as they do in the rat. This may be due to species-specific differences in the mossy fiber pathway. However, in the mouse, DYN levels are regulated by exogenous EB in the absence of ERα possibly via an ERβ-mediated pathway requiring new gene transcription.
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DOI：
10.1002/cne.22361
发表时间：
2010-07-15
期刊：
The Journal of comparative neurology
影响因子：
--
作者：
Mitterling KL;Spencer JL;Dziedzic N;Shenoy S;McCarthy K;Waters EM;McEwen BS;Milner TA
通讯作者：
Milner TA
影响因子：
2.7
作者：
Foy, Michael R.
通讯作者：
Foy, Michael R.
重复的游泳应力诱导阿片类阿片类药物介导的细胞外信号调节激酶1/2的激活。
影响因子：
1.7
作者：
Bruchas MR;Xu M;Chavkin C
通讯作者：
Chavkin C
DOI：
10.1126/science.1204622
发表时间：
2011-07-15
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Luo AH;Tahsili-Fahadan P;Wise RA;Lupica CR;Aston-Jones G
通讯作者：
Aston-Jones G
影响因子：
2.7
作者：
Day, M;Sung, A;Arias, R
通讯作者：
Arias, R