Multi-Omics Analyses Reveal the Molecular Mechanisms Underlying the Adaptation of Wheat (Triticum aestivum L.) to Potassium Deprivation.
Multi-Omics Analyses Reveal the Molecular Mechanisms Underlying the Adaptation of Wheat (Triticum aestivum L.) to Potassium Deprivation.
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多组学分析揭示了小麦 (Triticum aestivum L.) 缺钾适应的分子机制
DOI：
10.3389/fpls.2020.588994
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发表时间：
2020
影响因子：
5.6
通讯作者：
Xue C
中科院分区：
文献类型：
--
作者：
Zhao Y;Sun R;Liu H;Liu X;Xu K;Xiao K;Zhang S;Yang X;Xue C
钾（K）是调节植物生长和介导非生物胁迫反应所必需的。阐明植物对低钾胁迫反应的生物学机制，对于培育钾素吸收和利用效率高的新品种具有重要意义。在这项研究中，我们评估了543份小麦种质在苗期和成株期耐缺钾性不同的遗传变异程度。KN 9204和BN 207分别被鉴定为极耐和敏感的缺钾材料。加入暴露于正常和K-缺乏的条件下，之后，他们的根进行离子组学，转录组学和代谢组学分析。在缺钾条件下，KN 9204表现出更强的根系生长和维持较高的K浓度比BN 207。此外，根据转录组学和代谢组学分析，19，440个转录本和162个代谢物在两个加入物的根中差异丰富。基因表达和代谢谱的综合分析显示，在低钾胁迫下，KN 9204中有更多的基因（包括与离子稳态、细胞活性氧稳态和谷氨酸代谢途径相关的基因）表达上调。因此，这些候选基因对植物耐钾饥饿具有独特的调控作用。这些结果可能有助于进一步阐明小麦根系适应缺钾胁迫的分子变化。
Potassium (K) is essential for regulating plant growth and mediating abiotic stress responses. Elucidating the biological mechanism underlying plant responses to K-deficiency is crucial for breeding new cultivars with improved K uptake and K utilization efficiency. In this study, we evaluated the extent of the genetic variation among 543 wheat accessions differing in K-deficiency tolerance at the seedling and adult plant stages. Two accessions, KN9204 and BN207, were identified as extremely tolerant and sensitive to K-deficiency, respectively. The accessions were exposed to normal and K-deficient conditions, after which their roots underwent ionomic, transcriptomic, and metabolomic analyses. Under K-deficient conditions, KN9204 exhibited stronger root growth and maintained higher K concentrations than BN207. Moreover, 19,440 transcripts and 162 metabolites were differentially abundant in the roots of both accessions according to transcriptomic and metabolomic analyses. An integrated analysis of gene expression and metabolite profiles revealed that substantially more genes, including those related to ion homeostasis, cellular reactive oxygen species homeostasis, and the glutamate metabolic pathway, were up-regulated in KN9204 than in BN207 in response to low-K stress. Accordingly, these candidate genes have unique regulatory roles affecting plant K-starvation tolerance. These findings may be useful for further clarifying the molecular changes underlying wheat root adaptations to K deprivation.
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影响因子：
2.5
作者：
Steinfeld, Raphael;Herb, Jan T.;Sprengel, Rolf;Schaefer, Andreas T.;Fukunaga, Izumi
通讯作者：
Fukunaga, Izumi
两种对比西瓜基因型钾饥饿反应的比较转录组分析
影响因子：
4.3
作者：
Fan, Molin;Huang, Yuan;Bie, Zhilong
通讯作者：
Bie, Zhilong
一种用于大规模检测、鉴定和定量广泛目标代谢物的新型综合方法：在水稻代谢组学研究中的应用。
影响因子：
27.5
作者：
Chen, Wei;Gong, Liang;Luo, Jie
通讯作者：
Luo, Jie
DOI：
10.1074/mcp.ra117.000032
发表时间：
2017-11
期刊：
Molecular & cellular proteomics : MCP
影响因子：
--
作者：
Li G;Wu Y;Liu G;Xiao X;Wang P;Gao T;Xu M;Han Q;Wang Y;Guo T;Kang G
通讯作者：
Kang G
影响因子：
44.1
作者：
Held, Katrin;Pascaud, Francois;Kudla, Joerg
通讯作者：
Kudla, Joerg