Biopolymer system for cell recovery from microfluidic cell capture devices.
Biopolymer system for cell recovery from microfluidic cell capture devices.
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DOI：
10.1021/ac300190j
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发表时间：
2012-04-17
影响因子：
7.4
通讯作者：
Toner, Mehmet
中科院分区：
文献类型：
--
作者：
Shah, Ajay M.;Yu, Min;Nakamura, Zev;Ciciliano, Jordan;Ulman, Matthew;Kotz, Kenneth;Stott, Shannon L.;Maheswaran, Shyamala;Haber, Daniel A.;Toner, Mehmet
用于基于亲和力的细胞分离的微流体系统已成为从复杂材料中隔离特定细胞的一种有望的方法（即，在全血中循环肿瘤细胞），但是，这些技术仍缺乏可靠的方法，以使捕获的细胞无效地释放chips sirfulctions sirfulctiffictials chips。以下gel dissolution. This covalently crosslinked algorithm biopolymer system is stable in a wide variety of physiological solutions (including EDTA treated whole blood) and may be rapidly degraded via backbone cleavage with algorithmic lyase. The capture and release of EpCAM expressing cancer cells using this approach was found to have no significant effect on cell viability or proliferating potential and recovered cells were demonstrated to be compatible with downstream免疫染色和鱼类分析。
Microfluidic systems for affinity-based cell isolation have emerged as a promising approach for the isolation of specific cells from complex matrices (i.e., circulating tumor cells in whole blood). However, these technologies remain limited by the lack of reliable methods for the innocuous recovery of surface captured cells. Here, we present a biofunctional sacrificial hydrogel coating for microfluidic chips that enables the highly efficient release of isolated cells (99% ± 1%) following gel dissolution. This covalently crosslinked alginate biopolymer system is stable in a wide variety of physiologic solutions (including EDTA treated whole blood) and may be rapidly degraded via backbone cleavage with alginate lyase. The capture and release of EpCAM expressing cancer cells using this approach was found to have no significant effect on cell viability or proliferative potential and recovered cells were demonstrated to be compatible with downstream immunostaining and FISH analysis.
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影响因子：
158.5
作者：
Cristofanilli, M;Budd, GT;Hayes, DF
通讯作者：
Hayes, DF
DOI：
10.1021/la105016a
发表时间：
2011-04-05
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
--
作者：
Hatch A;Hansmann G;Murthy SK
通讯作者：
Murthy SK
影响因子：
4
作者：
Machaca K
通讯作者：
Machaca K
影响因子：
14
作者：
Ashton, Randolph S.;Banerjee, Akhilesh;Kane, Ravi S.
通讯作者：
Kane, Ravi S.
影响因子：
3.9
作者：
Cooperstein, Marta A.;Canavan, Heather E.
通讯作者：
Canavan, Heather E.