Quantum dots incorporated magnetic nanoparticles for imaging colon carcinoma cells.
Quantum dots incorporated magnetic nanoparticles for imaging colon carcinoma cells.
复制标题
DOI：
10.1186/1477-3155-11-28
复制
发表时间：
2013-08-17
影响因子：
10.2
通讯作者：
Park EY
中科院分区：
文献类型：
--
作者：
Ahmed S;Dong J;Yui M;Kato T;Lee J;Park EY
工程多功能纳米粒子（NPs）对生物医学科学产生了巨大的影响，在成像，传感和生物分离方面取得了进展。特别是，通过单粒子系统的光学和磁响应的组合使我们能够在临床环境中作为新型多模态分子成像造影剂。尽管基本的医学成像模式和重要的临床应用，只有少数纳米复合材料已开发出双重成像对比。一种基于层层自组装技术制备量子点磁性纳米粒子的新方法已经发展并用于癌细胞成像。在此，制备柠檬酸盐封端的带负电荷的Fe 3 O 4纳米颗粒，并用带正电荷的十六烷基三甲基溴化铵（CTAB）涂覆。然后，巯基封端的带负电荷的CdTe量子点与CTAB静电结合。对荧光磁性纳米粒子的形貌、光学和磁性进行了表征。将制备的FMNP另外与hCC 49抗体片段抗原结合（Fab）缀合，所述hCC 49抗体片段抗原结合（Fab）对LS 174 T癌细胞的TAG-72区域的唾液酸化糖链具有结合亲和力，所述hCC 49抗体片段抗原结合（Fab）是通过家蚕表达系统制备的，然后用于结肠癌细胞成像。所制备的纳米复合材料具有磁响应性和荧光性，同时可用于有效的细胞成像，光学传感和磁分离。透射电子显微镜（TEM）和动态光散射（DLS）结果表明，量子点的粒径约为50 nm，内部为磁性核，外部为CdTe核壳结构。制备的FMNP的细胞毒性测试表明在Vero细胞中的高活力。将抗肿瘤抗体偶联的纳米粒子成功地标记在结肠癌细胞（LS 174）上，并显示出对靶细胞的显著特异性。本报告展示了一个简单的合成CdTe量子点-Fe 3 O 4纳米粒子。所制备的FMNP的表面能够通过静电相互作用与单克隆抗体简单缀合。这一特性进一步扩展了它们作为细胞成像造影剂的体外应用。这种用新的荧光-磁纳米探针标记细胞用于活体检测的方法对各种生物医学应用都很有意义，并且已经证明了未来医学应用的潜力。
Engineered multifunctional nanoparticles (NPs) have made a tremendous impact on the biomedical sciences, with advances in imaging, sensing and bioseparation. In particular, the combination of optical and magnetic responses through a single particle system allows us to serve as novel multimodal molecular imaging contrast agents in clinical settings. Despite of essential medical imaging modalities and of significant clinical application, only few nanocomposites have been developed with dual imaging contrast. A new method for preparing quantum dots (QDs) incorporated magnetic nanoparticles (MNPs) based on layer-by-layer (LbL) self-assembly techniques have developed and used for cancer cells imaging. Here, citrate - capped negatively charged Fe3O4 NPs were prepared and coated with positively - charged hexadecyltrimethyl ammonium bromide (CTAB). Then, thiol - capped negatively charged CdTe QDs were electrostatically bound with CTAB. Morphological, optical and magnetic properties of the fluorescent magnetic nanoparticles (FMNPs) were characterized. Prepared FMNPs were additionally conjugated with hCC49 antibodies fragment antigen binding (Fab) having binding affinity to sialylated sugar chain of TAG-72 region of LS174T cancer cells, which was prepared silkworm expression system, and then were used for imaging colon carcinoma cells. The prepared nanocomposites were magnetically responsive and fluorescent, simultaneously that are useful for efficient cellular imaging, optical sensing and magnetic separation. Transmission electron microscopy (TEM) and dynamic light scattering (DLS) revealed that the particle size is around 50 nm in diameter with inner magnetic core and outer CdTe QDs core-shell structure. Cytotoxicity test of prepared FMNPs indicates high viability in Vero cells. NPs conjugated with anti cancer antibodies were successfully labeled on colon carcinoma cells (LS174) in vitro and showed significant specificity to target cells. The present report demonstrates a simple synthesis of CdTe QDs-Fe3O4 NPs. The surface of the prepared FMNPs was enabled simple conjugation to monoclonal antibodies by electrostatic interaction. This property further extended their in vitro applications as cellular imaging contrast agents. Such labeling of cells with new fluorescent-magneto nanoprobes for living detection is of interest to various biomedical applications and has demonstrated the potential for future medical use.
登录
查看更多内容
影响因子：
10.2
作者：
Verma NK;Crosbie-Staunton K;Satti A;Gallagher S;Ryan KB;Doody T;McAtamney C;MacLoughlin R;Galvin P;Burke CS;Volkov Y;Gun'ko YK
通讯作者：
Gun'ko YK
影响因子：
--
作者：
Ge Y;Zhang Y;He S;Nie F;Teng G;Gu N
通讯作者：
Gu N
影响因子：
3.5
作者：
Yang, Jaemoon;Lee, Jaewon;Haam, Seungjoo
通讯作者：
Haam, Seungjoo
影响因子：
4.8
作者：
Yoon, SO;Lee, TS;Hong, HJ
通讯作者：
Hong, HJ
影响因子：
15
作者：
Chang, In Pin;Hwang, Kuo Chu;Chiang, Chi-Shiun
通讯作者：
Chiang, Chi-Shiun