An ATP sensitive light addressable biosensor for extracellular monitoring of single taste receptor cell
An ATP sensitive light addressable biosensor for extracellular monitoring of single taste receptor cell
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一种用于单味觉受体细胞胞外监测的 ATP 敏感光可寻址生物传感器
DOI：
10.1007/s10544-012-9702-3
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发表时间：
2012-09
影响因子：
2.8
通讯作者：
Ping Wang
中科院分区：
文献类型：
--
作者：
Chunsheng Wu;Liping Du;Ling Zou;Luhang Zhao;Ping Wang
三磷酸腺苷（ATP）被认为是味蕾中用于味觉信号传递和处理的关键神经递质。以高灵敏度和特异性测量单个味觉受体细胞（TRC）分泌的ATP对于……是必不可少的
Adenosine triphosphate (ATP) is considered as the key neurotransmitter in taste buds for taste signal transmission and processing. Measurements of ATP secreted from single taste receptor cell (TRC) with high sensitivity and specificity are essential for investigating mechanisms underlying taste cell-to-cell communications. In this study, we presented an aptamer-based biosensor for the detection of ATP locally secreted from single TRC. ATP sensitive DNA aptamer was used as recognition element and its DNA competitor was served as signal transduction element that was covalently immobilized on the surface of light addressable potentiometric sensor (LAPS). Due to the light addressable capability of LAPS, local ATP secretion from single TRC can be detected by monitoring the working potential shifts of LAPS. The results show this biosensor can detect ATP with high sensitivity and specificity. It is demonstrated this biosensor can effectively detect the local ATP secretion from single TRC responding to tastant mixture. This biosensor could provide a promising new tool for the research of taste cell-to-cell communications as well as for the detection of local ATP secretion from other types of ATP secreting individual cells.
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影响因子：
12.6
作者：
Chunsheng Wu;Peihua Chen;Hui Yu;Qingjun Liu;Xiaolin Zong;H. Cai;Ping Wang
通讯作者：
Chunsheng Wu;Peihua Chen;Hui Yu;Qingjun Liu;Xiaolin Zong;H. Cai;Ping Wang
影响因子：
3.5
作者：
Clapp TR;Trubey KR;Vandenbeuch A;Stone LM;Margolskee RF;Chaudhari N;Kinnamon SC
通讯作者：
Kinnamon SC
影响因子：
4.8
作者：
Nutiu, R;Li, YF
通讯作者：
Li, YF
DOI：
10.1073/pnas.0611280104
发表时间：
2007-04-10
影响因子：
11.1
作者：
Huang, Yi-Jen;Maruyama, Yutaka;Roper, Stephen D.
通讯作者：
Roper, Stephen D.
影响因子：
12.6
作者：
Poghossian, A.;Abouzar, M. H.;Schoning, M. J.
通讯作者：
Schoning, M. J.