Enhanced biohydrogen production from corn stover hydrolyzate by pretreatment of two typical seed sludges

Enhanced biohydrogen production from corn stover hydrolyzate by pretreatment of two typical seed sludges
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通过预处理两种典型种子污泥提高玉米秸秆水解物的生物氢产量

DOI:
10.1016/j.ijhydene.2014.07.083
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发表时间:
2014-09
影响因子:
7.2
通讯作者:
Aijie Wang
Aijie Wang
中科院分区:
工程技术2区
文献类型:
--
作者:
Kun Zhang;Nanqi Ren;Aijie Wang

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以两种典型的种子污泥(河流底泥和厌氧颗粒污泥)为研究对象,采用热、超声波、紫外线、酸和碱5种预处理方法,研究了不同预处理方法对富集高效产氢菌和提高玉米秸秆水解液产氢能力的效果。结果表明,预处理工艺对河流沉积物的改善效果比厌氧颗粒污泥显著。在5种方案中,热预处理对底泥和厌氧颗粒污泥的产氢率均较高(分别为4.17 mmol H2/g利用糖和2.84 mmol H2/g利用糖)。紫外线和超声波预处理分别对河流沉积物和厌氧颗粒污泥有一定的预处理效果。在大多数情况下,预处理过程改变了可溶性代谢物的分布,使其产生更多的乙酸盐和更少的乙醇。微生物群落分析表明,热处理和超声波预处理分别会导致原始微生物群落发生显著和不显著的变化。除了经常检测到的土方工程外,沙雷氏菌属,和Klebsiellaspp.,梭菌属的一些种。和芽孢杆菌属(Bacillusspp.)可能是有效的H2生产者负责更好的H2生产性能。
Five individual pretreatment methods (heat, ultrasonic, ultraviolet, acid, and base) were performed on two typical seed sludges (river sediments and anaerobic granular sludge) to evaluate their effectiveness on enriching efficient hydrogen (H2)-producing bacteria and enhancing H2production using corn stover hydrolyzate. Results indicated that pretreatment processes caused more remarkable improvements for river sediments than anaerobic granular sludge. Among the five protocols, heat pretreatment reached high H2yield for both river sediments (4.17 mmol H2/g utilized sugar) and anaerobic granular sludge (2.84 mmol H2/g utilized sugar). Ultraviolet and ultrasonic pretreatments were conditionally effective for river sediments and anaerobic granular sludge, respectively. In most cases, pretreatment processes altered soluble metabolites distribution towards more acetate and less ethanol production. Microbial community analysis indicated that heat and ultrasonic pretreatments can respectively lead to significant and indistinctive change on original microbial community. Besides frequently detectedEscherichiaspp., Serratia spp., andKlebsiellaspp., some species of Clostridium spp. andBacillusspp. might be efficient H2producer responsible for better H2-producing performances.
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