Compliance profile of the human cornea as measured by atomic force microscopy.
Compliance profile of the human cornea as measured by atomic force microscopy.
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DOI：
10.1016/j.micron.2012.02.014
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发表时间：
2012-12
期刊：
影响因子：
2.4
通讯作者：
Murphy, Christopher J.
中科院分区：
文献类型：
--
作者：
Last, Julie A.;Thomasy, Sara M.;Croasdale, Christopher R.;Russell, Paul;Murphy, Christopher J.
准确确定人类角膜各层弹性模量的能力是设计更好的角膜假体的关键步骤。此外，弹性模量的知识将允许设计具有相关机械性能的基底，用于细胞行为的体外研究。之前，我们报道了人角膜前基底膜和后弹力层（分别与上皮细胞和内皮细胞接触的表面）的弹性模量值。通过获得鲍曼层和前基质的弹性模量值，我们已经完成了人类角膜基质元素的顺应性概况。原子力显微镜 (AFM) 用于确定弹性模量，弹性模量是组织刚度的量度，与顺应性成反比。组织的弹性响应允许使用赫兹方程进行分析，该模型提供压痕力和深度之间的关系，并且是尖端半径和基底模量的函数。角膜各层的弹性模量值为：7.5 ± 4.2 kPa（前基底膜）、109.8 ± 13.2 kPa（鲍曼层）、33.1 ± 6.1 kPa（前基质）和 50 ± 17.8 kPa（后弹力层）。这些结果表明，人类角膜每一层的生物物理特性（包括弹性模量）都是独特的，可能在维持体内平衡以及对治疗药物和疾病状态的反应中发挥作用。这些数据还将为改进角膜假体的设计和制造提供信息。
The ability to accurately determine the elastic modulus of each layer of the human cornea is a crucial step in the design of better corneal prosthetics. In addition, knowledge of the elastic modulus will allow design of substrates with relevant mechanical properties for in vitro investigations of cellular behavior. Previously, we have reported elastic modulus values for the anterior basement membrane and Descemet’s membrane of the human cornea, the surfaces in contact with the epithelial and endothelial cells, respectively. We have completed the compliance profile of the stromal elements of the human cornea by obtaining elastic modulus values for Bowman’s layer and the anterior stroma. Atomic force microscopy (AFM) was used to determine the elastic modulus, which is a measure of the tissue stiffness and is inversely proportional to the compliance. The elastic response of the tissue allows analysis with the Hertz equation, a model that provides a relationship between the indentation force and depth and is a function of the tip radius and the modulus of the substrate. The elastic modulus values for each layer of the cornea are: 7.5 ± 4.2 kPa (anterior basement membrane), 109.8 ± 13.2 kPa (Bowman’s layer), 33.1 ± 6.1 kPa (anterior stroma), and 50 ± 17.8 kPa (Descemet’s membrane). These results indicate that the biophysical properties, including elastic modulus, of each layer of the human cornea are unique and may play a role in the maintenance of homeostasis as well as in the response to therapeutic agents and disease states. The data will also inform the design and fabrication of improved corneal prosthetics.
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影响因子：
1.9
作者：
Jandt, KD
通讯作者：
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影响因子：
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作者：
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DOI：
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发表时间：
1987-01-01
期刊：
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影响因子：
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作者：
FISHER, RF
通讯作者：
FISHER, RF
影响因子：
12.6
作者：
Jayasuriya, AC;Ghosh, S;Kramer, P
通讯作者：
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