Comparative genomics and transcriptomics analyses reveal divergent lifestyle features of nematode endoparasitic fungus Hirsutella minnesotensis.
Comparative genomics and transcriptomics analyses reveal divergent lifestyle features of nematode endoparasitic fungus Hirsutella minnesotensis.
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比较基因组学和转录组学分析揭示线虫内寄生真菌明尼苏达被毛菌不同的生活方式特征
DOI：
10.1093/gbe/evu241
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发表时间：
2014-10-30
影响因子：
3.3
通讯作者：
Liu X
中科院分区：
文献类型：
--
作者：
Lai Y;Liu K;Zhang X;Zhang X;Li K;Wang N;Shu C;Wu Y;Wang C;Bushley KE;Xiang M;Liu X
明尼苏达被毛孢[Ophiocordycipitaceae（Hypocreales，Ascomycota）]是一种以分生孢子附着并穿透大豆胞囊线虫次级幼虫的优势内寄生真菌。其基因组进行了从头测序，并与肉座菌目（Hypocreales）中的5种昆虫病原真菌和Orbiliales（Ascomycota）中的3种捕食线虫真菌进行了比较。H. Minnesotensis基因全长51.4Mb，编码12,702个基因，其中转座因子占32%。系统基因组学分析表明，H. minnesotensis与肉座菌目昆虫病原真菌分离。H. Minnesotensis与昆虫病原真菌相似，具有较少的编码用于粘附的凝集素和用于纤维素降解的糖苷水解酶的基因，但与线虫捕获真菌不同，具有更多的用于蛋白质降解、信号转导和次级代谢的基因。这些结果表明，H. Minnesotensis的线虫内寄生机制与捕食线虫的真菌不同。转录组学分析的时间尺度寄生揭示了上调的凝集素，分泌的蛋白酶和次生代谢产物的生物合成的基因，可能是pueretically参与宿主表面粘附，角质层降解，和主机操纵。基因组和转录组分析提供了全面的了解线虫内寄生的进化和生活方式。
Hirsutella minnesotensis [Ophiocordycipitaceae (Hypocreales, Ascomycota)] is a dominant endoparasitic fungus by using conidia that adhere to and penetrate the secondary stage juveniles of soybean cyst nematode. Its genome was de novo sequenced and compared with five entomopathogenic fungi in the Hypocreales and three nematode-trapping fungi in the Orbiliales (Ascomycota). The genome of H. minnesotensis is 51.4 Mb and encodes 12,702 genes enriched with transposable elements up to 32%. Phylogenomic analysis revealed that H. minnesotensis was diverged from entomopathogenic fungi in Hypocreales. Genome of H. minnesotensis is similar to those of entomopathogenic fungi to have fewer genes encoding lectins for adhesion and glycoside hydrolases for cellulose degradation, but is different from those of nematode-trapping fungi to possess more genes for protein degradation, signal transduction, and secondary metabolism. Those results indicate that H. minnesotensis has evolved different mechanism for nematode endoparasitism compared with nematode-trapping fungi. Transcriptomics analyses for the time-scale parasitism revealed the upregulations of lectins, secreted proteases and the genes for biosynthesis of secondary metabolites that could be putatively involved in host surface adhesion, cuticle degradation, and host manipulation. Genome and transcriptome analyses provided comprehensive understanding of the evolution and lifestyle of nematode endoparasitism.
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秀丽隐杆线虫的角质层：其隔离和部分特征。
影响因子：
7.8
作者：
Cox, G N;Kusch, M;Edgar, R S
通讯作者：
Edgar, R S
影响因子：
4.5
作者：
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影响因子：
3.5
作者：
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通讯作者：
St Leger, RJ
影响因子：
3.4
作者：
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通讯作者：
Serrano, R
影响因子：
--
作者：
Collemare, Jerome;Billard, Alexis;Lebrun, Marc-Henri
通讯作者：
Lebrun, Marc-Henri