RGN-259 (thymosin β4) improves clinically important dry eye efficacies in comparison with prescription drugs in a dry eye model.
RGN-259 (thymosin β4) improves clinically important dry eye efficacies in comparison with prescription drugs in a dry eye model.
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DOI：
10.1038/s41598-018-28861-5
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发表时间：
2018-07-12
影响因子：
4.6
通讯作者：
Yang J
中科院分区：
文献类型：
--
作者：
Kim CE;Kleinman HK;Sosne G;Ousler GW;Kim K;Kang S;Yang J
本研究在小鼠干眼模型中评估了 RGN-259（胸腺素 β4）与环孢素 A (CsA)、地夸磷索 (DQS) 和 lifitegrast (LFA) 的临床活性。该模型是NOD.B10-H2b小鼠在30-40%的湿润环境中每天注射氢溴酸东莨菪碱10天。干燥应激后，所有药物在治疗 10 天后进行评估。 RGN-259 增加了泪液产生，与 CsA 不活跃时 DQS 和 LFA 处理的小鼠相似。 RGN-259 改善了角膜光滑度并减少了荧光素染色，与 LFA 组相似，而 CsA 和 DQS 处于非活性状态。 RGN-259 减少了角膜上皮脱离，DQS 和 LFA 显示出相似的活性，但 CsA 没有活性。 RGN-259 增加了结膜杯状细胞和粘蛋白的产生，与 CsA 所见的效果相当，而 DQS 和 LFA 则没有活性。 RGN-259 减少炎症因子的过度表达，与 CsA 和 LFA 相当，而 DQS 则无活性。 RGN-259 增加了粘蛋白的产生，与 CsA 观察到的结果相当，而 DQS 和 LFA 则没有活性。总之，RGN-259 促进了干眼小鼠模型中粘蛋白和杯状细胞的恢复，改善了角膜完整性，并减少了炎症，并且与处方治疗相同或更有效。
This study evaluated the clinical activity of RGN-259 (thymosin β4) in comparison with cyclosporine A (CsA), diquafosol (DQS), and lifitegrast (LFA) in a murine model of dry eye. The model was NOD.B10-H2b mice in a 30–40% humidified environment together with daily scopolamine hydrobromide injections for 10 days. After desiccation stress, all drugs were evaluated after 10 treatment days. RGN-259 increased tear production similar to that in the DQS- and LFA-treated mice while CsA was inactive. RGN-259 improved corneal smoothness and decreased fluorescein staining similar to that of LFA group while CsA and DQS were inactive. Corneal epithelial detachment was reduced by RGN-259, and DQS and LFA showed similar activity but the CsA was inactive. RGN-259 increased conjunctival goblet cells and mucin production comparable to that seen with CsA, while DQS and LFA were inactive. RGN-259 reduced the over-expression of inflammatory factors comparable to that with CsA and LFA, while DQS was inactive. RGN-259 increased mucin production comparable to that observed with CsA, while DQS and LFA were inactive. In conclusion, RGN-259 promoted recovery of mucins and goblet cells, improved corneal integrity, and reduced inflammation in a dry eye mouse model and was equal to or more effective than prescription treatments.
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影响因子：
4.6
作者：
Lee H;Kim CE;Ahn BN;Yang J
通讯作者：
Yang J
影响因子：
3.6
作者：
Galbis-Estrada, Carmen;Pinazo-Duran, Maria D.;Benitez-del-Castillo, Javier
通讯作者：
Benitez-del-Castillo, Javier
影响因子：
4.6
作者：
Kim CE;Lee JH;Yeon YK;Park CH;Yang J
通讯作者：
Yang J
影响因子：
3.3
作者：
Calonge, Margarita;Enriquez-de-Salamanca, Amalia;Corell, Alfredo
通讯作者：
Corell, Alfredo
影响因子：
4.4
作者：
Corrales, Rosa M.;Narayanan, Srihari;Calonge, Margarita
通讯作者：
Calonge, Margarita