Advanced light microscopy core facilities: Balancing service, science and career.
Advanced light microscopy core facilities: Balancing service, science and career.
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DOI：
10.1002/jemt.22648
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发表时间：
2016-06
影响因子：
2.5
通讯作者：
Avilov, Sergiy
中科院分区：
文献类型：
--
作者：
Ferrando-May, Elisa;Hartmann, Hella;Reymann, Juergen;Ansari, Nariman;Utz, Nadine;Fried, Hans-Ulrich;Kukat, Christian;Peychl, Jan;Liebig, Christian;Terjung, Stefan;Laketa, Vibor;Sporbert, Anje;Weidtkamp-Peters, Stefanie;Schauss, Astrid;Zuschratter, Werner;Avilov, Sergiy
关键词：
先进光学显微镜(ALM)的核心设备(CF)已成为生命科学研究不可或缺的支撑单位。它们的组织结构和技术特征相当不同，尽管它们追求的任务和提供的服务相似。因此，在整个欧洲，ALM-CFS的科学家们正在组建网络，以促进互动并讨论最佳实践模式。在这里，我们提出了由德国ALM-CFS网络、德国生物成像(GERBI)工作组详细阐述的ALM-CF手术建议。我们讨论了图像处理计划和仪器维护的技术问题，对ALM-CF的组织和管理提出了建议，提出了一个培训图像处理和分析用户的方案，并概述了当前的图像处理和分析资源。此外，我们还阐述了CFS为专业发展和职业生涯带来的新挑战和新机遇。虽然有些信息特别提到了德国的学术体系，但本文的大部分内容都是CFS在生命科学领域的普遍兴趣所在。Microsc.技术资源。2016年79：463-479。《作者显微镜研究与技术》由威利期刊出版公司出版。
Core Facilities (CF) for advanced light microscopy (ALM) have become indispensable support units for research in the life sciences. Their organizational structure and technical characteristics are quite diverse, although the tasks they pursue and the services they offer are similar. Therefore, throughout Europe, scientists from ALM‐CFs are forming networks to promote interactions and discuss best practice models. Here, we present recommendations for ALM‐CF operations elaborated by the workgroups of the German network of ALM‐CFs, German Bio‐Imaging (GerBI). We address technical aspects of CF planning and instrument maintainance, give advice on the organization and management of an ALM‐CF, propose a scheme for the training of CF users, and provide an overview of current resources for image processing and analysis. Further, we elaborate on the new challenges and opportunities for professional development and careers created by CFs. While some information specifically refers to the German academic system, most of the content of this article is of general interest for CFs in the life sciences. Microsc. Res. Tech. 79:463–479, 2016. © 2016 THE AUTHORS MICROSCOPY RESEARCH AND TECHNIQUE PUBLISHED BY WILEY PERIODICALS, INC.
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影响因子：
12.3
作者：
Carpenter AE;Jones TR;Lamprecht MR;Clarke C;Kang IH;Friman O;Guertin DA;Chang JH;Lindquist RA;Moffat J;Golland P;Sabatini DM
通讯作者：
Sabatini DM
影响因子：
2.8
作者：
Cole, Richard W.;Thibault, Marc;Brown, Claire M.
通讯作者：
Brown, Claire M.
影响因子：
48
作者：
de Chaumont, Fabrice;Dallongeville, Stephane;Olivo-Marin, Jean-Christophe
通讯作者：
Olivo-Marin, Jean-Christophe
影响因子：
48
作者：
Eliceiri, Kevin W.;Berthold, Michael R.;Goldberg, Ilya G.;Ibanez, Luis;Manjunath, B. S.;Martone, Maryann E.;Murphy, Robert F.;Peng, Hanchuan;Plant, Anne L.;Roysam, Badrinath;Stuurmann, Nico;Swedlow, Jason R.;Tomancak, Pavel;Carpenter, Anne E.
通讯作者：
Carpenter, Anne E.
DOI：
10.1006/meth.1999.0812
发表时间：
1999-08-01
期刊：
METHODS-A COMPANION TO METHODS IN ENZYMOLOGY
影响因子：
--
作者：
Zucker, RM;Price, OT
通讯作者：
Price, OT