Expression and Localization of TRK-Fused Gene Products in the Rat Brain and Retina.
Expression and Localization of TRK-Fused Gene Products in the Rat Brain and Retina.
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DOI：
10.1267/ahc.11015
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发表时间：
2012-02-29
影响因子：
2.4
通讯作者：
Tooyama I
中科院分区：
文献类型：
--
作者：
Maebayashi H;Takeuchi S;Masuda C;Makino S;Fukui K;Kimura H;Tooyama I
TRK 融合基因（人类 TFG，大鼠 Tfg）最初在人类乳头状甲状腺癌中被鉴定为 NTRK1 基因的嵌合形式。据报道，该基因产物（TFG）在调节磷酸酪氨酸特异性磷酸酶-1活性中发挥作用。然而，没有关于 Tfg 在大鼠组织中定位的信息。在这项研究中，我们使用逆转录聚合酶链反应（RT-PCR）研究了正常大鼠组织中 Tfg mRNA 的表达。我们还生产了针对 Tfg 基因产物的抗体，并检查了 TFG 在大鼠大脑和视网膜中的定位。 RT-PCR 实验表明，大鼠组织中表达了两种类型的 Tfg mRNA：传统形式的 Tfg (cTfg) 和新型变异形式视网膜 Tfg (rTfg)。 RT-PCR分析表明cTfg在大鼠组织中普遍表达，而rTfg主要在大脑和视网膜中表达。蛋白质印迹分析显示大鼠大脑中有两条分子量约为 30 kDa 和 50 kDa 的条带。免疫组织化学表明 TFG 蛋白主要由大脑中的神经元表达。在大鼠视网膜中，在视杆细胞层、视锥细胞层以及外丛状层中检测到强烈的 TFG 免疫反应性。
The TRK-fused gene (TFG in human, Tfg in rat) was originally identified in human papillary thyroid cancer as a chimeric form of the NTRK1 gene. It has been reported that the gene product (TFG) plays a role in regulating phosphotyrosine-specific phosphatase-1 activity. However, no information regarding the localization of Tfg in rat tissues is available. In this study, we investigated the expression of Tfg mRNA in normal rat tissues using reverse transcription-polymerase chain reaction (RT-PCR). We also produced an antibody against Tfg gene products and examined the localization of TFG in the rat brain and retina. The RT-PCR experiments demonstrated that two types of Tfg mRNA were expressed in rat tissues: the conventional form of Tfg (cTfg) and a novel variant form, retinal Tfg (rTfg). RT-PCR analyses demonstrated that cTfg was ubiquitously expressed in rat tissues, while rTfg was predominantly expressed in the brain and retina. Western blot analysis demonstrated two bands with molecular weights of about 30 kDa and 50 kDa in the rat brain. Immunohistochemistry indicated that TFG proteins were predominantly expressed by neurons in the brain. In the rat retina, intense TFG-immunoreactivity was detected in the layer of rods and cones and the outer plexiform layer.
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影响因子：
2.4
作者：
Nakahara H;Konishi Y;Beach TG;Yamada N;Makino S;Tooyama I
通讯作者：
Tooyama I
影响因子：
1.7
作者：
ABE, H;TOOYAMA, I;KIMURA, H
通讯作者：
KIMURA, H
影响因子：
8
作者：
Greco, A;Fusetti, L;Pierotti, MA
通讯作者：
Pierotti, MA
DOI：
10.1006/bbrc.1999.0417
发表时间：
1999-04-02
影响因子：
3.1
作者：
Mencinger, M;Aman, P
通讯作者：
Aman, P
影响因子：
4.4
作者：
Mencinger, M;Panagopoulos, I;Aman, P
通讯作者：
Aman, P