Yellow Fever Molecular Diagnosis Using Urine Specimens during Acute and Convalescent Phases of the Disease.
Yellow Fever Molecular Diagnosis Using Urine Specimens during Acute and Convalescent Phases of the Disease.
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DOI：
10.1128/jcm.00254-22
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发表时间：
2022-08-17
影响因子：
9.4
通讯作者：
Drumond, Betania Paiva
中科院分区：
文献类型：
--
作者：
de Rezende, Izabela Mauricio;Garcia Oliveira, Gabriela Fernanda;Costa, Thais Alkifeles;Khan, Aslam;Pereira, Leonardo Soares;Santos, Tayrine Araujo;Alves, Pedro Augusto;Calzavara-Silva, Carlos Eduardo;Martins-Filho, Olindo Assis;Teixeira-Carvalho, Andrea;LaBeaud, Angelle Desiree;Drumond, Betania Paiva
先前的研究已经证明，唾液和尿液中长期存在黄热病病毒(YFV)RNA，作为血清的替代品。为了调查尿液中YFV RNA的存在，我们使用RT-PCR对60份尿样进行了YFV筛查，这些尿样来自巴西最近爆发的YF疫情的急性和恢复期自然感染的黄热病患者(2017-2018)。15份急性期(症状出现后15 天)和4份恢复期(症状出现后69 天)尿样YFV聚合酶链式反应呈阳性。我们对7份尿样(5份在急性期收集，2份在YF恢复期收集)进行了YFV基因分型。基因分型表明这些样本中存在YFV南美I型。据我们所知，这是首次在距离症状出现这么远的地方(高达69个DPS)在尿液中检测到YFV野生型RNA，包括在YF感染的恢复期检测到YFV RNA。尿液中YFV RNA的检测是YFV感染的一个指标；然而，以尿液为样本的RT-PCR结果应谨慎解读，因为阴性结果不排除YFV感染的可能性。由于病毒期以外的检测时间可能较长，尿样与血清学检测、流行病学调查和临床评估相结合，可为实验室YF诊断提供更长的诊断窗口。
Prior studies have demonstrated prolonged presence of yellow fever virus (YFV) RNA in saliva and urine as an alternative to serum. To investigate the presence of YFV RNA in urine, we used RT-PCR for YFV screening in 60 urine samples collected from a large cohort of naturally infected yellow fever (YF) patients during acute and convalescent phases of YF infection from recent YF outbreaks in Brazil (2017 to 2018). Fifteen urine samples from acute phase infection (up to 15 days post-symptom onset) and four urine samples from convalescent phase infection (up to 69 days post-symptom onset), were YFV PCR-positive. We genotyped YFV detected in seven urine samples (five collected during the acute phase and two collected during the YF convalescent phase). Genotyping indicated the presence of YFV South American I genotype in these samples. To our knowledge, this is the first report of wild-type YFV RNA detection in the urine this far out from symptom onset (up to 69 DPS), including YFV RNA detection during the convalescent phase of YF infection. The detection of YFV RNA in urine is an indicative of YFV infection; however, the results of RT-PCR using urine as sample should be interpreted with care, since a negative result does not exclude the possibility of YFV infection. With a possible prolonged period of detection beyond the viremic phase, the use of urine samples coupled with serological tests, epidemiologic inquiry, and clinical assessment could provide a longer diagnostic window for laboratory YF diagnosis.
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影响因子：
3.8
作者：
Sacchetto L;Silva NIO;Rezende IM;Arruda MS;Costa TA;de Mello ÉM;Oliveira GFG;Alves PA;de Mendonça VE;Stumpp RGAV;Prado AIA;Paglia AP;Perini FA;Lacerda Nogueira M;Kroon EG;de Thoisy B;Trindade GS;Drumond BP
通讯作者：
Drumond BP
影响因子：
3.8
作者：
Sacchetto L;Drumond BP;Han BA;Nogueira ML;Vasilakis N
通讯作者：
Vasilakis N
影响因子：
5.4
作者：
Giovanetti, Marta;Lima de Mendonca, Marcos Cesar;Bispo de Filippis, Ana Maria
通讯作者：
Bispo de Filippis, Ana Maria
影响因子：
5.5
作者：
Martinez, Miguel J.;Vilella, Anna;Hayes, Edward B.
通讯作者：
Hayes, Edward B.
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro