Extra-virgin olive oil phenols block cell cycle progression and modulate chemotherapeutic toxicity in bladder cancer cells.
Extra-virgin olive oil phenols block cell cycle progression and modulate chemotherapeutic toxicity in bladder cancer cells.
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DOI：
10.3892/or.2016.5150
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发表时间：
2016-12
期刊：
影响因子：
4.2
通讯作者：
Lendaro E
中科院分区：
文献类型：
--
作者：
Coccia A;Mosca L;Puca R;Mangino G;Rossi A;Lendaro E
流行病学数据表明，每天食用特级初榨橄榄油（EVOO）是地中海地区的一种常见饮食习惯，可降低某些类型癌症的发病率，特别是膀胱肿瘤。本研究的目的是评估从EVOO中提取的多酚对膀胱癌（BCa）的抗增殖活性，并阐明触发细胞死亡的生物学机制。此外，我们还评估了低剂量特级初榨橄榄油提取物（EVOOE）调节紫杉醇或丝裂霉素（两种用于治疗不同类型癌症的药物）体外活性的能力。结果表明，EVOOE能明显抑制T24和5637 BCa细胞的增殖和克隆形成能力，并呈剂量依赖性。此外，EVOOE处理后的细胞周期分析显示，两种细胞系在G2/M期有丝分裂前明显生长停滞，随后仅在T24细胞中诱导凋亡。值得注意的是，丝裂霉素C和EVOOE的同时处理由于ROS产生的抑制而降低了药物的细胞毒性。相反，与单独的紫杉醇相比，用紫杉醇和多酚提取物共同处理T24细胞在每个测试浓度下强烈增加了凋亡性细胞死亡。我们的研究结果支持流行病学证据，表明橄榄油消费对健康有益，并可能成为开发新抗癌策略的起点。
Epidemiological data indicate that the daily consumption of extra-virgin olive oil (EVOO), a common dietary habit of the Mediterranean area, lowers the incidence of certain types of cancer, in particular bladder neoplasm. The aim of the present study was to evaluate the antiproliferative activity of polyphenols extracted from EVOO on bladder cancer (BCa), and to clarify the biological mechanisms that trigger cell death. Furthermore, we also evaluated the ability of low doses of extra-virgin olive oil extract (EVOOE) to modulate the in vitro activity of paclitaxel or mitomycin, two antineoplastic drugs used in the management of different types of cancer. Our results showed that EVOOE significantly inhibited the proliferation and clonogenic ability of T24 and 5637 BCa cells in a dose-dependent manner. Furthermore, cell cycle analysis after EVOOE treatment showed a marked growth arrest prior to mitosis in the G2/M phase for both cell lines, with the subsequent induction of apoptosis only in the T24 cells. Notably, simultaneous treatment of mitomycin C and EVOOE reduced the drug cytotoxicity due to inhibition of ROS production. Conversely, the co-treatment of T24 cells with paclitaxel and the polyphenol extract strongly increased the apoptotic cell death at each tested concentration compared to paclitaxel alone. Our results support the epidemiological evidence indicating that olive oil consumption exerts health benefits and may represent a starting point for the development of new anticancer strategies.
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影响因子：
8.4
作者：
Brinkman, Maree T.;Buntinx, Frank;Zeegers, Maurice P.
通讯作者：
Zeegers, Maurice P.
DOI：
10.1080/01635581.2014.922204
发表时间：
2014-01-01
影响因子：
2.9
作者：
Coccia, Andrea;Bastianelli, Daniela;Lendaro, Eugenio
通讯作者：
Lendaro, Eugenio
影响因子：
8.8
作者：
Masci, Alessandra;Coccia, Andrea;Cesa, Stefania
通讯作者：
Cesa, Stefania
影响因子：
4
作者：
Goodison, Steve;Rosser, Charles J.;Urquidi, Virginia
通讯作者：
Urquidi, Virginia
DOI：
10.1016/j.bbrc.2005.06.161
发表时间：
2005-09-02
影响因子：
3.1
作者：
Hamdi, HK;Castellon, R
通讯作者：
Castellon, R