Armillaria mellea Symbiosis Drives Metabolomic and Transcriptomic Changes in Polyporus umbellatus Sclerotia.
Armillaria mellea Symbiosis Drives Metabolomic and Transcriptomic Changes in Polyporus umbellatus Sclerotia.
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蜜环菌共生驱动猪苓菌核的代谢组学和转录组学变化
DOI：
10.3389/fmicb.2021.792530
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发表时间：
2021
影响因子：
5.2
通讯作者：
Guo SX
中科院分区：
文献类型：
--
作者：
Xing YM;Li B;Liu L;Li Y;Yin SX;Yin SC;Chen J;Guo SX
硬核是伞形蓼的药用部位，具有利尿、保肾等重要作用，其主要活性成分为甾体和多糖。菌核只有与蜜环菌共生后才能生长发育。本研究基于非靶向UPLC-MS方法，对菌核分离腔壁感染部分（QR）与未感染部分（对照组，CK）进行了系统代谢组学分析，寻找并鉴定差异代谢物。推导了伞藻菌核中特征甾体的生物合成途径，并采用高效液相色谱法检测了QR组和CK组麦角甾醇、多porusterone A和B的含量。此外，我们还利用实时荧光定量PCR检测了类固醇生物合成途径相关基因的表达模式。结果表明，通过UPLC-MS代谢组学分析，共鉴定出258个碎片化评分大于45分、高分辨率质量的真菌代谢物，其中两组间存在118个差异表达代谢物（DEMs）。代谢途径表明，在蜜蜂侵染伞伞菌菌核过程中，类固醇、脂肪酸和碳水化合物具有活性并富集。与对照组相比，QR组麦角甾醇、多porusterone A和B的含量分别增加了32.2、75.0和20.0%。qRT-PCR分析显示，在麦角甾醇生物合成的最后步骤中起重要作用的C-8甾醇异构酶（ERG2）、甾醇C-24甲基转移酶（ERG6）和甾醇22去饱和酶（ERG5）等一系列酶在QR组中均呈上调模式。全面的代谢组学和转录组学信息将为今后进一步研究蜜铃虫感染伞形假蝇的菌核形成机制奠定基础。
Sclerotia, the medicinal part of Polyporus umbellatus, play important roles in diuresis and renal protection, with steroids and polysaccharides as the main active ingredients. The sclerotia grow and develop only after symbiosis with Armillaria sp. In this study, a systematic metabolomics based on non-targeted UPLC-MS method was carried out between the infected part of the separated cavity wall of the sclerotia (QR) and the uninfected part (the control group, CK) to find and identify differential metabolites. The biosynthetic pathway of characteristic steroids in sclerotia of P. umbellatus was deduced and the content of ergosterol, polyporusterone A and B in the QR and CK groups were detected with the High Performance Liquid Chromatography (HPLC). Furthermore, the expression patterns of putative genes associated with steroid biosynthesis pathway were also performed with quantitative real-time PCR. The results showed that a total of 258 metabolites originated from fungi with the fragmentation score more than 45 and high resolution mass were identified, based on UPLC-MS metabolomic analysis, and there were 118 differentially expressed metabolites (DEMs) between both groups. The metabolic pathways indicated that steroids, fatty acid and carbohydrate were active and enriched during P. umbellatus sclerotia infected by A. mellea. The content of ergosterol, polyporusterone A and B in the QR group increased by 32.2, 75.0, and 20.0%, in comparison to that of the control group. The qRT-PCR analysis showed that series of enzymes including C-8 sterol isomerase (ERG2), sterol C-24 methyltransferase (ERG6) and sterol 22-desaturase (ERG5), which played important roles in the final steps of ergosterol biosynthesis, all presented up-regulated patterns in the QR group in P. umbellatus. The comprehensive metabolomic and transcriptomic information will contribute to further study concerning the mechanisms of P. umbellatus sclerotial formation infected by A. mellea in the future.
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影响因子：
2.9
作者：
Wen, Jing;Yang, Lina;Xiong, Zhili
通讯作者：
Xiong, Zhili
影响因子：
1.4
作者：
Bandara, Asanka R.;Rapior, Sylvie;Hyde, Kevin D.
通讯作者：
Hyde, Kevin D.
蜜环菌感染猪苓防御反应相关基因的转录组分析
影响因子：
4.6
作者：
Liu MM;Xing YM;Zhang DW;Guo SX
通讯作者：
Guo SX
影响因子：
1.6
作者：
Qiao YL;Xiang WQ;Liu F;Jin S
通讯作者：
Jin S
影响因子：
5.8
作者：
Koressaar, Triinu;Remm, Maido
通讯作者：
Remm, Maido