A Wox3-patterning module organizes planar growth in grass leaves and ligules.
A Wox3-patterning module organizes planar growth in grass leaves and ligules.
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DOI：
10.1038/s41477-023-01405-0
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发表时间：
2023-05
期刊：
影响因子：
18
通讯作者：
Scanlon, Michael J.
中科院分区：
文献类型：
--
作者：
Satterlee, James W.;Evans, Lukas J.;Conlon, Brianne R.;Conklin, Phillip;Martinez-Gomez, Jesus;Yen, Jeffery R.;Wu, Hao;Sylvester, Anne W.;Specht, Chelsea D.;Cheng, Jie;Johnston, Robyn;Coen, Enrico;Scanlon, Michael J.
草叶由茎尖分生组织外围的一圈原始初始细胞发育而来，茎尖分生组织是一个产生植物茎的所有器官的器官干细胞池。成熟时，草叶是一个扁平的带状器官，包括围绕茎的近端支持鞘和远端光合叶片。叶鞘和叶片被一个铰链状的耳廓和叶舌分开，叶舌是一种从叶片正面（顶部）生长的表皮衍生组织的边缘。舌叶和耳廓共同构成了草叶特有的形态学特征。了解草叶及其毗邻叶舌的平面生长是如何受基因控制的，可以深入了解它们的进化起源。在这里，我们使用单细胞rna测序分析来鉴定存在于玉米叶原基边缘的“边缘”细胞类型。叶缘细胞具有独特的特性，并与增殖的舌叶细胞共享转录特征，这表明叶片和舌叶具有共同的发育遗传程序。此外，我们发现边缘功能受遗传冗余的wuschellike homeobox3 （WOX3）转录因子调控。玉米Wox3基因的高阶突变大大降低了叶片宽度，破坏了叶舌的生长和图案。总之，这些发现说明了边缘结构域在玉米叶片和叶舌平面生长过程中的可推广应用，并提出了草叶舌同源性作为叶鞘边缘远端延伸的简约模型。草叶舌包括叶基和光合叶片之间的边界；它们的同源性已经争论了200多年。细胞特异性基因表达分析表明，舌叶与叶基边缘同源。
Grass leaves develop from a ring of primordial initial cells within the periphery of the shoot apical meristem, a pool of organogenic stem cells that generates all of the organs of the plant shoot. At maturity, the grass leaf is a flattened, strap-like organ comprising a proximal supportive sheath surrounding the stem and a distal photosynthetic blade. The sheath and blade are partitioned by a hinge-like auricle and the ligule, a fringe of epidermally derived tissue that grows from the adaxial (top) leaf surface. Together, the ligule and auricle comprise morphological novelties that are specific to grass leaves. Understanding how the planar outgrowth of grass leaves and their adjoining ligules is genetically controlled can yield insight into their evolutionary origins. Here we use single-cell RNA-sequencing analyses to identify a ‘rim’ cell type present at the margins of maize leaf primordia. Cells in the leaf rim have a distinctive identity and share transcriptional signatures with proliferating ligule cells, suggesting that a shared developmental genetic programme patterns both leaves and ligules. Moreover, we show that rim function is regulated by genetically redundant Wuschel-like homeobox3 (WOX3) transcription factors. Higher-order mutations in maize Wox3 genes greatly reduce leaf width and disrupt ligule outgrowth and patterning. Together, these findings illustrate the generalizable use of a rim domain during planar growth of maize leaves and ligules, and suggest a parsimonious model for the homology of the grass ligule as a distal extension of the leaf sheath margin. Grass ligules comprise the boundary between the leaf base and the photosynthetic blade; their homology has been debated for over 200 years. Cell-specific gene expression analyses suggest that ligules are homologous to the margins of the leaf base.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
12.3
作者：
Efroni I;Ip PL;Nawy T;Mello A;Birnbaum KD
通讯作者：
Birnbaum KD
DOI：
10.1111/nph.16837
发表时间：
2021-01
期刊：
The New phytologist
影响因子：
--
作者：
Liu X;Bourgault R;Galli M;Strable J;Chen Z;Feng F;Dong J;Molina I;Gallavotti A
通讯作者：
Gallavotti A
影响因子：
11.6
作者：
Johnston, Robyn;Wang, Minghui;Scanlon, Michael J.
通讯作者：
Scanlon, Michael J.
影响因子：
14.9
作者：
Birnbaum, Kenneth D.;Kussell, Edo
通讯作者：
Kussell, Edo