Identification of candidate genes controlling oil content by combination of genome-wide association and transcriptome analysis in the oilseed crop Brassica napus
Identification of candidate genes controlling oil content by combination of genome-wide association and transcriptome analysis in the oilseed crop Brassica napus
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通过全基因组关联和转录组分析相结合鉴定油料作物甘蓝型油菜中控制含油量的候选基因
DOI：
10.1186/s13068-019-1557-x
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发表时间：
2019-09
影响因子：
6.3
通讯作者：
Li Nannan
中科院分区：
文献类型：
--
作者：
Xiao Zhongchun;Zhang Chao;Tang Fang;Yang Bo;Zhang Liyuan;Liu Jingsen;Huo Qiang;Wang Shufeng;Li Shengting;Wei Lijuan;Du Hai;Qu Cunmin;Lu Kun;Li Jiana;Li Nannan
提高油菜种子含油量是油菜育种的重要目标之一。然而，甘蓝型油菜（B. napus）仍然鲜为人知。为了确定油含量相关的基因，全基因组关联研究（GWAS）进行了588 accessibs.ResultsHigh-throughput基因组重测序导致385,692高质量的单核苷酸多态性（SNP）与次要等位基因频率（MAF）0.05。结果表明，共检测到17个与种子含油量显著相关的位点，其中12个SNP分布在A3（11个位点）和A1（1个位点）染色体上，5个新的显著SNP分别分布在C5（1个位点）和C7（4个位点）染色体上。随后，我们的特点是差异表达基因（DEGs）之间的种子和角果果皮上的主花期和一级分支极高和低含油量的加入（HO和LO）。共鉴定出64个与脂质代谢相关的DEG，其中14个参与三酰甘油（TAG）的生物合成和组装。此外，我们分析了高油和低油含量之间质体中从头脂肪酸生物合成、内质网（ER）中TAG组装和脂滴包装的关键基因转录水平的差异。GWAS和转录组分析的组合揭示了位于显著SNP的置信区间内的7个候选基因。本研究结果为利用分子标记育种提高大豆含油量提供了有价值的信息。油菜。
BackgroundIncreasing seed oil content is one of the most important targets for rapeseed (Brassica napus) breeding. However, genetic mechanisms of mature seed oil content inBrassica napus(B. napus) remain little known. To identify oil content-related genes, a genome-wide association study (GWAS) was performed using 588 accessions.ResultsHigh-throughput genome resequencing resulted in 385,692 high-quality single nucleotide polymorphism (SNPs) with a minor allele frequency (MAF) 0.05. We identified 17 loci that were significantly associated with seed oil content, among which 12 SNPs were distributed on the A3 (11 loci) and A1 (one loci) chromosomes, and five novel significant SNPs on the C5 (one loci) and C7 (four loci) chromosomes, respectively. Subsequently, we characterized differentially expressed genes (DEGs) between the seeds and silique pericarps on main florescences and primary branches of extremely high- and low-oil content accessions (HO and LO). A total of 64 lipid metabolism-related DEGs were identified, 14 of which are involved in triacylglycerols (TAGs) biosynthesis and assembly. Additionally, we analyzed differences in transcription levels of key genes involved in de novo fatty acid biosynthesis in the plastid, TAGs assembly and lipid droplet packaging in the endoplasmic reticulum (ER) between high- and low-oil contentB. napusaccessions.ConclusionsThe combination of GWAS and transcriptome analyses revealed seven candidate genes located within the confidence intervals of significant SNPs. Current findings provide valuable information for facilitating marker-based breeding for higher seed oil content inB. napus.
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影响因子：
5.3
作者：
通讯作者：
--
DOI：
10.1093/dnares/dsu002
发表时间：
2014-08
期刊：
DNA research : an international journal for rapid publication of reports on genes and genomes
影响因子：
--
作者：
Li F;Chen B;Xu K;Wu J;Song W;Bancroft I;Harper AL;Trick M;Liu S;Gao G;Wang N;Yan G;Qiao J;Li J;Li H;Xiao X;Zhang T;Wu X
通讯作者：
Wu X
影响因子：
3.7
作者：
Wang X;Wang H;Long Y;Li D;Yin Y;Tian J;Chen L;Liu L;Zhao W;Zhao Y;Yu L;Li M
通讯作者：
Li M
甘蓝型油菜种子含油量的母体控制：长角果壁光合作用的作用
影响因子：
7.2
作者：
Hua, Wei;Li, Rong-Jun;Wang, Han-Zhong
通讯作者：
Wang, Han-Zhong
影响因子：
5.8
作者：
Bradbury, Peter J.;Zhang, Zhiwu;Buckler, Edward S.
通讯作者：
Buckler, Edward S.