Antiproliferative effects of different plant parts of Panax notoginseng on SW480 human colorectal cancer cells.
Antiproliferative effects of different plant parts of Panax notoginseng on SW480 human colorectal cancer cells.
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DOI：
10.1002/ptr.2383
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发表时间：
2009-01
影响因子：
7.2
通讯作者：
Yuan, Chun-Su
中科院分区：
文献类型：
--
作者：
Wang, Chong-Zhi;Xie, Jing-Tian;Fishbein, Anna;Aung, Han H.;He, Hui;Mehendale, Sangeeta R.;He, Tong-Chuan;Du, Wei;Yuan, Chun-Su
对三七不同部位的化学成分及对人结肠癌细胞株SW 480的抑制作用进行了研究。采用高效液相色谱法测定了三七根、根茎、花和浆果提取物中皂苷的含量。不同部位皂苷的含量和比例不同。使用细胞计数法，评价抗增殖作用，结果表明，0.05-1.0 mg/mL的所有四种提取物对癌细胞显示出浓度相关的抗增殖作用。花提取物的抑制作用最强，在1.0mg/mL浓度下，抑制率达93.1%（p < 0.01）。对三七中主要皂苷类成分三七皂苷R1、三七皂苷Rb 1、Rb 3和Rg 1的抗增殖作用进行了评价，观察到的主要成分的作用支持了提取物的药理活性。流式细胞仪检测三七提取物对SW 480细胞周期及凋亡的影响。诺氟沙星提取物可使细胞阻滞于S期和G2/M期。观察到三七提取物具有显著的细胞凋亡诱导活性，其中花提取物具有最有效的效果，支持抗增殖作用。
The chemical constituents and antiproliferative effects on SW480 human colorectal cancer cells of different plant parts of P. notoginseng were evaluated. The contents of saponins in extracts from root, rhizome, flower and berry of P. notoginseng were determined using high performance liquid chromatography. The contents and proportions of saponins were different among the four plant parts. Using the cell counting method, the antiproliferative effects were evaluated and the results indicated all four extracts, at 0.05–1.0 mg/mL, showed concentration-related antiproliferative effects on the cancer cells. The flower extract had stronger effects compared with the other three extracts; at 1.0 mg/mL, it inhibited the cell growth by 93.1% (p < 0.01). The antiproliferative effects of major saponins in notoginseng, notoginsenoside R1, ginsenosides Rb1, Rb3 and Rg1, were also evaluated, and the observed effects of major constituents support the pharmacological activities of extracts. The effects of notoginseng extracts on cell cycle and apoptosis of SW480 cells were determined using flow cytometry. Notoginseng extract can arrest the cells in S and G2/M phases. Remarkably apoptosis induction activities of notoginseng extracts were observed with the flower extract possessing the most potent effect, supporting the antiproliferative effect.
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影响因子：
1.7
作者：
Washida, D;Kitanaka, S
通讯作者：
Kitanaka, S
影响因子：
254.7
作者：
Jemal, A;Tiwari, RC;Thun, MJ
通讯作者：
Thun, MJ
影响因子：
2.9
作者：
Yang, ZG;Sun, HX;Ye, YP
通讯作者：
Ye, YP
影响因子：
5.7
作者：
Chen, FD;Wu, MC;Ou, YH
通讯作者：
Ou, YH
影响因子：
6.1
作者：
Khan, IA
通讯作者：
Khan, IA