Polysaccharides from Artocarpus heterophyllus Lam. (jackfruit) pulp improves intestinal barrier functions of high fat diet-induced obese rats.
Polysaccharides from Artocarpus heterophyllus Lam. (jackfruit) pulp improves intestinal barrier functions of high fat diet-induced obese rats.
复制标题
来自木菠萝的多糖。
DOI：
10.3389/fnut.2022.1035619
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发表时间：
2022
影响因子：
5
通讯作者：
Tan, Lehe
中科院分区：
文献类型：
--
作者：
Zeng, Shunjiang;Cao, Jun;Chen, Yuzi;Li, Chuan;Wu, Gang;Zhu, Kexue;Chen, Xiaoai;Xu, Fei;Liu, Qibing;Tan, Lehe
关键词：
多糖由于其在减轻氧化损伤、炎症和益生菌作用方面的有效性而显示出对肠屏障功能的保护作用。菠萝蜜多糖的作用机制尚不清楚。果肉（菠萝蜜，JFP-Ps）对肠屏障功能的影响。本研究旨在探讨JFP-Ps对高脂饮食诱导的肥胖大鼠肠道屏障功能的影响。进行H&E染色和生化分析以测量肠道的病理和炎症状态以及氧化损伤。通过RT-qPCR和蛋白质印迹分析与肠道健康和炎症相关的基因和蛋白质的表达。结果表明，JFP-Ps通过降低粪便pH值和增加粪便含水量，促进排便，改善肠道理化环境。JFP-Ps还减轻了结肠的氧化损伤，减轻了肠道结肠炎症，并调节了小肠中的血糖转运。此外，JFP-Ps通过修复肠粘膜损伤和增加粘液层厚度来改善肠道生理状态。此外，JFP-Ps还下调了炎症因子TNF-α、IL-6的表达，上调了游离脂肪酸受体GPR 41、GPR 43和紧密连接蛋白occludin的表达。这些结果表明，JFP-Ps通过增强肠道的生物屏障、黏膜屏障、免疫屏障和机械屏障功能，激活SCFAs-GPR 41/GPR 43相关信号通路，对肠道功能具有保护作用。JFP-Ps可能被用作改善人类肠道健康的有前途的植物化学物质。
Polysaccharides show protective effects on intestinal barrier function due to their effectiveness in mitigating oxidative damage, inflammation and probiotic effects. Little has been known about the effects of polysaccharides from Artocarpus heterophyllus Lam. pulp (jackfruit, JFP-Ps) on intestinal barrier function. This study aimed to investigate the effects of JFP-Ps on intestinal barrier function in high fat diet-induced obese rats. H&E staining and biochemical analysis were performed to measure the pathological and inflammatory state of the intestine as well as oxidative damage. Expression of the genes and proteins associated with intestinal health and inflammation were analyzed by RT-qPCR and western blots. Results showed that JFP-Ps promoted bowel movements and modified intestinal physiochemical environment by lowering fecal pH and increasing fecal water content. JFP-Ps also alleviated oxidative damage of the colon, relieved intestinal colonic inflammation, and regulated blood glucose transport in the small intestine. In addition, JFP-Ps modified intestinal physiological status through repairing intestinal mucosal damage and increasing the thickness of the mucus layer. Furthermore, JFP-Ps downregulated the inflammatory genes (TNF-α, IL-6) and up-regulated the free fatty acid receptors (GPR41 and GPR43) and tight junction protein (occludin). These results revealed that JFP-Ps showed a protective effect on intestinal function through enhancing the biological, mucosal, immune and mechanical barrier functions of the intestine, and activating SCFAs-GPR41/GPR43 related signaling pathways. JFP-Ps may be used as a promising phytochemical to improve human intestinal health.
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