Efficacy of FimA antibody and clindamycin in silkworm larvae stimulated with Porphyromonas gulae.
Efficacy of FimA antibody and clindamycin in silkworm larvae stimulated with Porphyromonas gulae.
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DOI：
10.1080/20002297.2021.1914499
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发表时间：
2021-04-25
影响因子：
4.5
通讯作者：
Matsumoto-Nakano M
中科院分区：
文献类型：
--
作者：
Yoshida S;Inaba H;Nomura R;Murakami M;Yasuda H;Nakano K;Matsumoto-Nakano M
目的：古氏卟啉单胞菌是一种主要的牙周病原菌，其菌毛可根据fimA基因的多样性分为A、B、C三种类型。具有C型菌毛的古氏肺孢子虫菌株以前被证明比其他类型的毒力更强。在本研究中，我们通过构建重组FimA(RFimA)表达载体，进一步检测了古氏并殖吸虫菌毛对宿主的毒力，并提高了纯化蛋白的抗体水平。方法和结果：小剂量感染古氏肺吸虫C型幼虫后204h内全部死亡，而A型和B型幼虫未见死亡。在FimA类型中，注射rFimAC型幼虫的存活率显著下降，而注射A型和B型rFimA型幼虫的存活率均在50%以上。克林霉素以剂量依赖的方式抑制C型家蚕的生长，提高家蚕的存活率。最后，C型rFimA特异性fic抗血清延长了感染古氏肺吸虫C株或注射rFimA C型蛋白刺激的家蚕幼虫的存活。结论：C型菌毛具有增强细菌致病作用的潜力，克林霉素和抗C型rFimA抗体均可有效抑制C型菌毛的毒性。这是首次报道利用古氏肺孢子虫建立家蚕感染模型进行毒性评估。
Objective: Porphyromonas gulae, a major periodontal pathogen in animals, possesses fimbriae that have been classified into three genotypes (A, B, C) based on the diversity of fimA genes encoding fimbrillin protein (FimA). P. gulae strains with type C fimbriae were previously shown to be more virulent than other types. In this study, we further examined the host toxicity mediated by P. gulae fimbriae by constructing recombinant FimA (rFimA) expression vectors for each genotype and raised antibodies to the purified proteins. Methods and Results: All larvae died within 204 h following infection with P. gulae type C at the low-dose infection, whereas type A and B did not. Among fimA types, the survival rates of the larvae injected with rFimA type C were remarkably decreased, while the survival rates of the larvae injected with rFimA type A and type B were greater than 50%. Clindamycin treatment inhibited the growth of type C strains in a dose-dependent manner, resulting in an increased rate of silkworm survival. Finally, type C rFimA-specific antiserum prolonged the survival of silkworm larvae stimulated by infection with P. gulae type C strain or injection of rFimA type C protein. Conclusion: These results suggested that type C fimbriae have high potential for enhancement of bacterial pathogenesis, and that both clindamycin and anti-type C rFimA-specific antibodies are potent inhibitors of type C fimbriae-induced toxicity. This is the first report to establish a silkworm infection model using P. gulae for toxicity assessment.
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影响因子：
2.3
作者：
Casadevall, Arturo;Pirofski, Liise-anne
通讯作者：
Pirofski, Liise-anne
影响因子：
--
作者：
Inaba, H.;Nakano, K.;Amano, A.
通讯作者：
Amano, A.
影响因子：
4.2
作者：
Jiminez JA;Uwiera TC;Douglas Inglis G;Uwiera RR
通讯作者：
Uwiera RR
影响因子：
1
作者：
Kato, Yukio;Shirai, Mitsuyuki;Asai, Fumitoshi
通讯作者：
Asai, Fumitoshi
影响因子：
3.3
作者：
Hamada, Nobushiro;Takahashi, Yusuke;Umemoto, Toshio
通讯作者：
Umemoto, Toshio