Diversity of Xenorhabdus and Photorhabdus spp. and their symbiotic entomopathogenic nematodes from Thailand.
Diversity of Xenorhabdus and Photorhabdus spp. and their symbiotic entomopathogenic nematodes from Thailand.
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DOI：
10.1371/journal.pone.0043835
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Chantratita N
中科院分区：
文献类型：
--
作者：
Thanwisai A;Tandhavanant S;Saiprom N;Waterfield NR;Ke Long P;Bode HB;Peacock SJ;Chantratita N
Xenorhabdus和光habdus是昆虫病原线虫（EPNs）的细菌共生体。本研究从泰国各地分离并鉴定了Xenorhabdus和Photorhabdus及其相关的线虫共生体，并对其系统发育多样性进行了研究。利用gallera诱捕技术从土壤样品中分离epn。从EPNs中培养细菌并根据recA序列进行基因分型。根据28S rDNA序列和内部转录间隔区进行鉴定。在泰国13个省的159个地点共收集了795个土壤样本。从10个省份分离的126份EPNs中检出Xenorhabdus （n = 69）或photohabdus spp （n = 57）。系统发育分析将69株Xenorhabdus分离物分为4个类群。第1、2和3组由52、13和1株与X. stockiae相关的分离株组成，第4组由3株与X. miraniensis相关的分离株组成。与stockiae相关分离株的EPN宿主为S. websteri， X. miraniensis的EPN宿主为S. khoisanae。光habdus属植物分别为P. luminescens （n = 56）和P. asymbiotica （n = 1）。系统发育分析将发光假体分为5个类群。第1组和第2组分别有45株和8株被定义为海南亚种和阿库尔斯蒂亚种。其中1株与海南种和阿库尔斯蒂菌有亲缘关系，2株与劳蒙地菌有亲缘关系，1株为病原菌P. asymbitica亚种。南极光。籼稻是光habdus主要的EPN寄主。本研究揭示了泰国Xenorhabdus和Photorhabdus的遗传多样性，并描述了epn与其细菌共生体之间的新联系。
Xenorhabdus and Photorhabdus spp. are bacterial symbionts of entomopathogenic nematodes (EPNs). In this study, we isolated and characterized Xenorhabdus and Photorhabdus spp. from across Thailand together with their associated nematode symbionts, and characterized their phylogenetic diversity. EPNs were isolated from soil samples using a Galleria-baiting technique. Bacteria from EPNs were cultured and genotyped based on recA sequence. The nematodes were identified based on sequences of 28S rDNA and internal transcribed spacer regions. A total of 795 soil samples were collected from 159 sites in 13 provinces across Thailand. A total of 126 EPNs isolated from samples taken from 10 provinces were positive for Xenorhabdus (n = 69) or Photorhabdus spp. (n = 57). Phylogenetic analysis separated the 69 Xenorhabdus isolates into 4 groups. Groups 1, 2 and 3 consisting of 52, 13 and 1 isolates related to X. stockiae, and group 4 consisting of 3 isolates related to X. miraniensis. The EPN host for isolates related to X. stockiae was S. websteri, and for X. miraniensis was S. khoisanae. The Photorhabdus species were identified as P. luminescens (n = 56) and P. asymbiotica (n = 1). Phylogenenic analysis divided P. luminescens into five groups. Groups 1 and 2 consisted of 45 and 8 isolates defined as subspecies hainanensis and akhurstii, respectively. One isolate was related to hainanensis and akhurstii, two isolates were related to laumondii, and one isolate was the pathogenic species P. asymbiotica subsp. australis. H. indica was the major EPN host for Photorhabdus. This study reveals the genetic diversity of Xenorhabdus and Photorhabdus spp. and describes new associations between EPNs and their bacterial symbionts in Thailand.
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DOI：
10.1590/s0074-02762008000200005
发表时间：
2008-03-01
期刊：
Memórias do Instituto Oswaldo Cruz
影响因子：
--
作者：
Dolinski, Claudia;Kamitani, Fernando L;Winter, Carlos E
通讯作者：
Winter, Carlos E
影响因子：
10.7
作者：
TAMURA, K;NEI, M
通讯作者：
NEI, M
影响因子：
3.4
作者：
Kanga, Francoise Ngo;Waeyenberge, Lieven;Moens, Maurice
通讯作者：
Moens, Maurice
影响因子：
3.4
作者：
Nguyen, Khuong B.;Ambros Ginarte, Carmen M.;Harakava, Ricardo
通讯作者：
Harakava, Ricardo
影响因子：
2.1
作者：
Sharma, S;Waterfield, N;ffrench-Constant, R
通讯作者：
ffrench-Constant, R