Nanoplasmonic pillars engineered for single exosome detection.
Nanoplasmonic pillars engineered for single exosome detection.
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DOI：
10.1371/journal.pone.0202773
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Raphael MP
中科院分区：
文献类型：
--
作者：
Raghu D;Christodoulides JA;Christophersen M;Liu JL;Anderson GP;Robitaille M;Byers JM;Raphael MP
外来体是分泌的纳米囊泡，其掺入蛋白质和核酸，从而使细胞间通讯的多功能途径成为可能。越来越多的人认识到它们在发育、伤口愈合和疾病进展等基本过程中发挥的关键作用，但由于它们的分子含量不均匀和体内浓度低，它们的检测和表征仍然是一个挑战。在这项工作中，我们结合联合收割机纳米和微制造技术，用于创建针对单个外泌体检测的纳米传感阵列。椭圆形纳米等离子体传感器被制造成容纳至多一个外来体，并在真实的时间内单独成像，从而能够以高度多路复用的几何形状无标记地记录数字响应。这种方法的结果在一个三个数量级的灵敏度比以前报道的实时，多路复用平台的改善。每个纳米传感器都被提升到一个石英纳米柱的顶部，最大限度地减少了不必要的非特异性底物结合贡献。该方法通过检测MCF 7乳腺癌细胞分泌的外泌体来验证。我们证明了越来越多的数字和随机性的响应的二次采样的纳米传感器的数量从四百减少到一个。
Exosomes are secreted nanovesicles which incorporate proteins and nucleic acids, thereby enabling multifunctional pathways for intercellular communication. There is an increasing appreciation of the critical role they play in fundamental processes such as development, wound healing and disease progression, yet because of their heterogeneous molecular content and low concentrations in vivo, their detection and characterization remains a challenge. In this work we combine nano- and microfabrication techniques for the creation of nanosensing arrays tailored toward single exosome detection. Elliptically–shaped nanoplasmonic sensors are fabricated to accommodate at most one exosome and individually imaged in real time, enabling the label-free recording of digital responses in a highly multiplexed geometry. This approach results in a three orders of magnitude sensitivity improvement over previously reported real-time, multiplexed platforms. Each nanosensor is elevated atop a quartz nanopillar, minimizing unwanted nonspecific substrate binding contributions. The approach is validated with the detection of exosomes secreted by MCF7 breast adenocarcinoma cells. We demonstrate the increasingly digital and stochastic nature of the response as the number of subsampled nanosensors is reduced from four hundred to one.
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影响因子：
16
作者：
Lötvall J;Hill AF;Hochberg F;Buzás EI;Di Vizio D;Gardiner C;Gho YS;Kurochkin IV;Mathivanan S;Quesenberry P;Sahoo S;Tahara H;Wauben MH;Witwer KW;Théry C
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Théry C
影响因子：
3.4
作者：
Raphael, Marc P.;Christodoulides, Joseph A.;Byers, Jeff M.
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Byers, Jeff M.
影响因子：
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作者：
Su, Judith
通讯作者：
Su, Judith
影响因子：
7.4
作者：
Raphael, Marc P.;Christodoulides, Joseph A.;Byers, Jeff M.
通讯作者：
Byers, Jeff M.
影响因子：
21.3
作者：
Skog, Johan;Wuerdinger, Tom;van Rijn, Sjoerd;Meijer, Dimphna H.;Gainche, Laura;Sena-Esteves, Miguel;Curry, William T., Jr.;Carter, Bob S.;Krichevsky, Anna M.;Breakefield, Xandra O.
通讯作者：
Breakefield, Xandra O.