Organization and morphology of calcitonin gene-related peptide-immunoreactive axons in the whole mouse stomach.
Organization and morphology of calcitonin gene-related peptide-immunoreactive axons in the whole mouse stomach.
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DOI：
10.1002/cne.25519
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发表时间：
2023-11
影响因子：
2.5
通讯作者：
Cheng, Zixi (Jack)
中科院分区：
文献类型：
--
作者：
Ma, Jichao;Nguyen, Duyen;Madas, Jazune;Bizanti, Ariege;Mistareehi, Anas;Kwiat, Andrew M.;Chen, Jin;Lin, Mabelle;Christie, Richard;Hunter, Peter;Heal, Maci;Baldwin, Shane;Tappan, Susan;Furness, John B.;Powley, Terry L.;Cheng, Zixi (Jack)
关键词：
伤害性传入轴突支配胃并将信号发送到大脑和脊髓。外周伤害性传入可以用多种标记物（例如，P物质[SP]和降钙素基因相关肽[CGRP]）。最近，我们研究了地形组织和形态的SP免疫反应（SP-IR）轴突在整个小鼠胃肌层。然而，CGRP-IR轴突的分布和形态结构仍不清楚。我们使用免疫组织化学标记和应用成像技术的组合，包括共聚焦和蔡司成像仪M2显微镜，Neurolucida 360跟踪，并将轴突跟踪数据整合到三维胃支架中，以表征整个小鼠胃肌层中的CGRP-IR轴突和终末。结果发现：（1）CGRP阳性神经元在腹侧和背侧胃内均形成广泛的终末网络。(2)CGRP-IR轴突密集地支配血管。(3)CGRP-IR轴突与纵肌和环肌平行。一些轴突成角度地穿过肌肉层。(4)它们还与单个肌间神经节神经元形成静脉曲张的终端接触。(5)CGRP免疫反应出现在背根和迷走结状神经节中DiI标记的胃投射神经元，表明CGRP免疫反应轴突为内脏传入轴突。(6)CGRP-IR轴突在胃中不与酪氨酸羟化酶或囊泡乙酰胆碱转运体轴突共定位，表明CGRP-IR轴突不是内脏传出轴突。(7)追踪CGRP-IR轴突并整合到3D胃支架中。本研究首次以细胞/轴突/静脉曲张的尺度提供了全胃肌层CGRP-IR轴突神经支配的地形分布图。
Nociceptive afferent axons innervate the stomach and send signals to the brain and spinal cord. Peripheral nociceptive afferents can be detected with a variety of markers (e.g., substance P [SP] and calcitonin gene-related peptide [CGRP]). We recently examined the topographical organization and morphology of SP-immunoreactive (SP-IR) axons in the whole mouse stomach muscular layer. However, the distribution and morphological structure of CGRP-IR axons remain unclear. We used immunohistochemistry labeling and applied a combination of imaging techniques, including confocal and Zeiss Imager M2 microscopy, Neurolucida 360 tracing, and integration of axon tracing data into a 3D stomach scaffold to characterize CGRP-IR axons and terminals in the whole mouse stomach muscular layers. We found that: (1) CGRP-IR axons formed extensive terminal networks in both ventral and dorsal stomachs. (2) CGRP-IR axons densely innervated the blood vessels. (3) CGRP-IR axons ran in parallel with the longitudinal and circular muscles. Some axons ran at angles through the muscular layers. (4) They also formed varicose terminal contacts with individual myenteric ganglion neurons. (5) CGRP-IR occurred in DiI-labeled gastric-projecting neurons in the dorsal root and vagal nodose ganglia, indicating CGRP-IR axons were visceral afferent axons. (6) CGRP-IR axons did not colocalize with tyrosine hydroxylase or vesicular acetylcholine transporter axons in the stomach, indicating CGRP-IR axons were not visceral efferent axons. (7) CGRP-IR axons were traced and integrated into a 3D stomach scaffold. For the first time, we provided a topographical distribution map of CGRP-IR axon innervation of the whole stomach muscular layers at the cellular/axonal/varicosity scale.
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影响因子：
2.5
作者：
BARTHO, L;KOCZAN, G;SZOLCSANYI, J
通讯作者：
SZOLCSANYI, J
影响因子：
2
作者：
Domeneghini, C;Radaelli, G;Dolera, M
通讯作者：
Dolera, M
影响因子：
2.4
作者：
Di Natale, Madeleine R.;Patten, Lauren;Furness, John B.
通讯作者：
Furness, John B.
影响因子：
5.8
作者：
Achanta, Sirisha;Gorky, Jonathan;Schwaber, James S.
通讯作者：
Schwaber, James S.
DOI：
10.1007/978-1-4939-0897-4_3
发表时间：
2014-01-01
期刊：
MICROBIAL ENDOCRINOLOGY: THE MICROBIOTA-GUT-BRAIN AXIS IN HEALTH AND DISEASE
影响因子：
--
作者：
Furness, John B.;Callaghan, Brid P.;Cho, Hyun-Jung
通讯作者：
Cho, Hyun-Jung