A member of the TERMINAL FLOWER 1/CENTRORADIALIS gene family controls sprout growth in potato tubers.
A member of the TERMINAL FLOWER 1/CENTRORADIALIS gene family controls sprout growth in potato tubers.
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DOI：
10.1093/jxb/ery387
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发表时间：
2019-02-05
影响因子：
6.9
通讯作者：
Taylor MA
中科院分区：
文献类型：
--
作者：
Morris WL;Alamar MC;Lopez-Cobollo RM;Castillo Cañete J;Bennett M;Van der Kaay J;Stevens J;Kumar Sharma S;McLean K;Thompson AJ;Terry LA;Turnbull CGN;Bryan GJ;Taylor MA
转基因实验表明，TERMINAL FLOWER 1/CENTRORADIALIS与马铃薯块茎芽在贮藏过程中的生长有关，并与细胞分裂素和ABA水平的调节有关。马铃薯块茎芽休眠破裂后过早出芽是马铃薯生产中主要的商业问题，它造成马铃薯品质损失，降低了块茎的适销性。马铃薯块茎早熟的防治方法之一是培育休眠时间较长或发芽速度较慢的马铃薯品种。我们最近在马铃薯二倍体群体中发现了几个与块茎芽生长相关的数量性状位点（qtl）。在目前的研究中，我们的目标是表征马铃薯3号染色体上影响块茎芽快速生长的最大qtl之一的候选基因。该QTL的底层是一个编码终端花1/CENTRORADIALIS同源基因（PGSC0003DMG400014322）的基因。在这里，我们使用转基因方法来操纵CEN家族成员在马铃薯四倍体基因型(cv。拿破仑情史)。我们证明了操纵StCEN表达的明显效果，表达水平降低与芽生长速度增加相关，过表达系的芽生长速度低于对照。与StCEN不同表达水平相关的是不同水平的脱落酸和细胞分裂素，这可能与控制植物顶端分生组织中生长调节剂的水平有关。
Transgenic experiments demonstrate that TERMINAL FLOWER 1/CENTRORADIALIS is associated with potato tuber sprout growth during storage, and with the regulation of cytokinin and ABA levels. Potato tuber bud dormancy break followed by premature sprouting is a major commercial problem which results in quality losses and decreased tuber marketability. An approach to controlling premature tuber sprouting is to develop potato cultivars with a longer dormancy period and/or reduced rate of sprout growth. Our recent studies using a potato diploid population have identified several quantitative trait loci (QTLs) that are associated with tuber sprout growth. In the current study, we aim to characterize a candidate gene associated with one of the largest effect QTLs for rapid tuber sprout growth on potato chromosome 3. Underlying this QTL is a gene encoding a TERMINAL FLOWER 1/CENTRORADIALIS homologue (PGSC0003DMG400014322). Here, we use a transgenic approach to manipulate the expression level of the CEN family member in a potato tetraploid genotype (cv. Désirée). We demonstrate a clear effect of manipulation of StCEN expression, with decreased expression levels associated with an increased rate of sprout growth, and overexpressing lines showing a lower rate of sprout growth than controls. Associated with different levels of StCEN expression were different levels of abscisic acid and cytokinins, implying a role in controlling the levels of plant growth regulators in the apical meristem.
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影响因子：
11.6
作者：
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通讯作者：
Goto, Koji
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影响因子：
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影响因子：
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