Transcriptome analysis of Pseudostellaria heterophylla in response to the infection of pathogenic Fusarium oxysporum.
Transcriptome analysis of Pseudostellaria heterophylla in response to the infection of pathogenic Fusarium oxysporum.
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太子参响应病原性尖孢镰刀菌感染的转录组分析
DOI：
10.1186/s12870-017-1106-3
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发表时间：
2017-09-18
影响因子：
5.3
通讯作者：
Lin W
中科院分区：
文献类型：
--
作者：
Qin X;Wu H;Chen J;Wu L;Lin S;Khan MU;Boorboori MR;Lin W
背景：太子参为石竹科多年生草本植物，是具有较高药效学价值的中草药之一。可用于治疗脾虚、食欲不振、病后虚弱、自汗等症状。我们前期的研究发现，太子参连续单一栽培会导致根际微环境的恶化。病原真菌尖孢镰刀菌的专化型。结果：利用尖孢霉菌侵染异叶松组培苗，采用RNA测序的方法研究了尖孢霉在三个不同侵染阶段的反应过程。我们通过从头组装获得了127,725个转录本和47,655个不同的非基因，并获得了25,882个非基因的详细注释信息。京都百科全书的基因和基因组途径分析和实时定量PCR结果表明，植物-病原菌相互作用途径中的钙信号系统和WRKY转录因子可能在反应过程中发挥重要作用，所有WRKY转录因子基因被分为三种不同的类型。结论：本研究部分揭示了尖孢镰刀菌在根际土壤中种群爆炸的可能分子机制和信号响应过程，有助于揭示尖孢镰刀菌在连作单作问题中的作用。
Background:Pseudostellaria heterophylla (P. heterophylla), a herbaceous perennial, belongs to Caryophyllaceae family and is one of the Chinese herbal medicine with high pharmacodynamic value. It can be used to treat the spleen deficiency, anorexia, weakness after illness and spontaneous perspiration symptoms. Our previous study found that consecutive monoculture of Pseudostellaria heterophylla could lead to the deterioration of the rhizosphere microenvironment. The specialized forms of pathogenic fungus Fusarium oxysporum f.Sp. heterophylla (F. oxysporum) in rhizosphere soils of P. heterophylla plays an important role in the consecutive monoculture of P. heterophylla.Results:In this study, F. oxysporum was used to infect the tissue culture plantlets of P. heterophylla to study the responding process at three different infection stages by using RNA-sequencing. We obtained 127,725 transcripts and 47,655 distinct unigenes by de novo assembly and obtained annotated information in details for 25,882 unigenes. The Kyoto Encyclopedia of Genes and Genomes pathway analysis and the real-time quantitative PCR results suggest that the calcium signal system and WRKY transcription factor in the plant-pathogen interaction pathway may play an important role in the response process, and all of the WRKY transcription factor genes were divided into three different types. Moreover, we also found that the stimulation of F. oxysporum may result in the accumulation of some phenolics in the plantlets and the programmed cell death of the plantlets.Conclusions:This study has partly revealed the possible molecular mechanism of the population explosion of F. oxysporum in rhizosphere soils and signal response process, which can be helpful in unraveling the role of F. oxysporum in consecutive monoculture problems of P. heterophylla.
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影响因子：
20.5
作者：
Stael S;Kmiecik P;Willems P;Van Der Kelen K;Coll NS;Teige M;Van Breusegem F
通讯作者：
Van Breusegem F
影响因子：
4.4
作者：
Tsanakas GF;Manioudaki ME;Economou AS;Kalaitzis P
通讯作者：
Kalaitzis P
影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
DOI：
10.1126/science.1171647
发表时间：
2009-05-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Boller T;He SY
通讯作者：
He SY
影响因子：
3.7
作者：
Sebastiana M;Vieira B;Lino-Neto T;Monteiro F;Figueiredo A;Sousa L;Pais MS;Tavares R;Paulo OS
通讯作者：
Paulo OS