Tensile properties of craniofacial tendons in the mature and aged zebrafish.
Tensile properties of craniofacial tendons in the mature and aged zebrafish.
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DOI：
10.1002/jor.22847
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发表时间：
2015-06
影响因子：
2.8
通讯作者：
Galloway, Jenna L.
中科院分区：
文献类型：
--
作者：
Shah, Rishita R.;Nerurkar, Nandan L.;Wang, Calvin C.;Galloway, Jenna L.
斑马鱼斑马鱼是研究发育、再生生物学和人类疾病的强大模型。然而，该系统对肌腱和韧带等承重组织的分析受到限制。这主要是由于技术限制无法准确测量其机械性能。在这里，我们提出了一种定制拉伸测试系统，该系统将纳米牛顿级的力施加到长度小至 1 毫米的斑马鱼肌腱。肌腱特性与哺乳动物肌腱非常相似，包括应力应变非线性和与哺乳动物数据密切相关的线性模量 (515±152 MPa)。此外，用于描述肌腱力学的简单指数本构定律成功地适用于斑马鱼肌腱；相关的材料常数与哺乳动物肌腱的文献值一致。最后，成熟和老年斑马鱼的比较显示机械功能随着年龄的增长而显着下降。根据指数本构模型，年龄相关的变化主要是由非线性的减少引起的（例如胶原蛋白卷曲或纤维募集的变化）。这些发现证明了斑马鱼作为研究健康和疾病肌腱生物力学模型的实用性。此外，这些发现表明肌腱机械行为在脊椎动物中高度保守。
The zebrafish Danio rerio is a powerful model for the study of development, regenerative biology, and human disease. However, the analysis of load-bearing tissues such as tendons and ligaments has been limited in this system. This is largely due to technical limitations that preclude accurate measurement of their mechanical properties. Here, we present a custom tensile testing system that applies nano-Newton scale forces to zebrafish tendons as small as 1 mm in length. Tendon properties were remarkably similar to mammalian tendons, including stress-strain nonlinearity and a linear modulus (515±152 MPa) that aligned closely with mammalian data. Additionally, a simple exponential constitutive law used to describe tendon mechanics was successfully fit to zebrafish tendons; the associated material constants agreed with literature values for mammalian tendons. Finally, mature and aged zebrafish comparisons revealed a significant decline in mechanical function with age. Based on the exponential constitutive model, age related changes were primarily caused by a reduction in nonlinearity (e.g. changes in collagen crimp or fiber recruitment). These findings demonstrate the utility of zebrafish as a model to study tendon biomechanics in health and disease. Moreover, these findings suggest that tendon mechanical behavior is highly conserved across vertebrates.
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影响因子：
64.5
作者：
López-Otín C;Blasco MA;Partridge L;Serrano M;Kroemer G
通讯作者：
Kroemer G
DOI：
10.1016/j.clinbiomech.2009.12.007
发表时间：
2010-05
期刊：
Clinical biomechanics (Bristol, Avon)
影响因子：
--
作者：
Abramowitch SD;Zhang X;Curran M;Kilger R
通讯作者：
Kilger R
影响因子：
3.8
作者：
Elliott, DM;Robinson, PS;Soslowsky, LJ
通讯作者：
Soslowsky, LJ
DOI：
10.1115/1.2898732
发表时间：
2008-04-01
影响因子：
1.7
作者：
Goh, K. L.;Holmes, D. F.;Wess, T. J.
通讯作者：
Wess, T. J.
DOI：
10.1098/rspb.1972.0019
发表时间：
1972-01-01
期刊：
PROCEEDINGS OF THE ROYAL SOCIETY SERIES B-BIOLOGICAL SCIENCES
影响因子：
--
作者：
DIAMANT, J;ARRIDGE, RGC;KELLER, A
通讯作者：
KELLER, A