Worldwide sequence conservation of transmission-blocking vaccine candidate Pvs230 in Plasmodium vivax.
Worldwide sequence conservation of transmission-blocking vaccine candidate Pvs230 in Plasmodium vivax.
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DOI：
10.1016/j.vaccine.2011.04.028
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发表时间：
2011-06-10
期刊：
影响因子：
5.5
通讯作者：
Tsuboi, Takafumi
中科院分区：
文献类型：
--
作者：
Doi, Masanori;Tanabe, Kazuyuki;Tachibana, Shin-Lchiro;Hamai, Meiko;Tachibana, Mayumi;Mita, Toshihiro;Yagi, Masanori;Zeyrek, Fadile Yildiz;Ferreira, Marcelo U.;Ohmae, Hiroshi;Kaneko, Akira;Randrianarivelojosia, Milijaona;Sattabongkot, Jetsumon;Cao, Ya-Ming;Horii, Toshihiro;Torii, Motomi;Tsuboi, Takafumi
关键词：
恶性疟原虫配子体表面蛋白Pfs 230是阻断疟疾传播疫苗的主要候选蛋白。间日疟原虫具有Pfs 230的直向同源物（Pvs 230），然而，迄今为止还没有关于Pvs 230的任何方面的研究。为了研究Pvs 230是否可以作为间日疟传播阻断疫苗，我们对Pvs 230基因（pvs 230：PVX_003905）进行了进化和群体遗传学分析。我们的分析Pvs 230和它的直系同源物在7个疟原虫物种揭示了两个独特的部分：种间可变部分（IVP）含有物种特异性寡肽重复的N-末端和7.5 kb的种间保守部分（ICP）含有14个富含半胱氨酸的结构域。在猴疟原虫中，Pvs 230与其直系同源物Pks 230和Pcys 230密切相关。对113个来自世界各地的pvs 230基因序列的分析表明，pvs 230非重复区（θπ = 0.00118）的核苷酸多样性非常低，有77个多态位点，其中40个位点发生了氨基酸替换。在pvs 230上观察到纯化选择而不是平衡选择的特征。功能和/或结构约束可能限制pvs 230中多态性的水平。观察到的有限的多态性在pvs 230应地面利用Pvs 230作为一个有效的传播阻断疫苗。
Pfs230, surface protein of gametocyte/gamete of the human malaria parasite, Plasmodium falciparum, is a prime candidate of malaria transmission-blocking vaccine. P. vivax has an ortholog of Pfs230 (Pvs230), however, there has been no study in any aspects on Pvs230 to date. To investigate whether Pvs230 can be a vivax malaria transmission-blocking vaccine, we performed evolutionary and population genetic analysis of the Pvs230 gene (pvs230: PVX_003905). Our analysis of Pvs230 and its orthologs in seven Plasmodium species revealed two distinctive parts: an interspecies variable part (IVP) containing species-specific oligopeptide repeats at the N-terminus and a 7.5 kb interspecies conserved part (ICP) containing 14 cysteine-rich domains. Pvs230 was closely related to its orthologs, Pks230 and Pcys230, in monkey malaria parasites. Analysis of 113 pvs230 sequences obtained from worldwide, showed that nucleotide diversity is remarkably low in the non-repeat 8-kb region of pvs230 (θπ = 0.00118) with 77 polymorphic nucleotide sites, 40 of which resulting in amino acid replacements. A signature of purifying selection but not of balancing selection was seen on pvs230. Functional and/or structural constraints may limit the level of polymorphism in pvs230. The observed limited polymorphism in pvs230 should ground for utilization of Pvs230 as an effective transmission-blocking vaccine.
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影响因子：
64.8
作者：
Carlton, Jane M.;Adams, John H.;Silva, Joana C.;Bidwell, Shelby L.;Lorenzi, Hernan;Caler, Elisabet;Crabtree, Jonathan;Angiuoli, Samuel V.;Merino, Emilio F.;Amedeo, Paolo;Cheng, Qin;Coulson, Richard M. R.;Crabb, Brendan S.;del Portillo, Hernando A.;Essien, Kobby;Feldblyum, Tamara V.;Fernandez-Becerra, Carmen;Gilson, Paul R.;Gueye, Amy H.;Guo, Xiang;Kang'a, Simon;Kooij, Taco W. A.;Korsinczky, Michael;Meyer, Esmeralda V. -S.;Nene, Vish;Paulsen, Ian;White, Owen;Ralph, Stuart A.;Ren, Qinghu;Sargeant, Tobias J.;Salzberg, Steven L.;Stoeckert, Christian J.;Sullivan, Steven A.;Yamamoto, Marcio M.;Hoffman, Stephen L.;Wortman, Jennifer R.;Gardner, Malcolm J.;Galinski, Mary R.;Barnwell, John W.;Fraser-Liggett, Claire M.
通讯作者：
Fraser-Liggett, Claire M.
影响因子：
3.6
作者：
Healer, J;Murphy, V;Batchelor, A
通讯作者：
Batchelor, A
影响因子：
2.4
作者：
Healer, J;McGuinness, D;Riley, E
通讯作者：
Riley, E
影响因子：
2.4
作者：
Martinez, O;Suarez, CF;Patarroyo, MA
通讯作者：
Patarroyo, MA
影响因子：
2.4
作者：
Gomez, A.;Suarez, C. F.;Patarroyo, M. A.
通讯作者：
Patarroyo, M. A.