Effects of Transanal Irrigation on Gut Microbiota in Pediatric Patients with Spina Bifida.
Effects of Transanal Irrigation on Gut Microbiota in Pediatric Patients with Spina Bifida.
复制标题
DOI：
10.3390/jcm10020224
复制
发表时间：
2021-01-10
影响因子：
3.9
通讯作者：
Yoshimura N
中科院分区：
文献类型：
--
作者：
Furuta A;Suzuki Y;Takahashi R;Jakobsen BP;Kimura T;Egawa S;Yoshimura N
最近使用基于16 S rRNA的微生物群分析的研究表明便秘患者的肠道微生物群失调。本研究旨在探讨脊柱裂（SB）患者经肛门冲洗（TAI）后肠道菌群的变化。在16名6-17岁的SB患者和10名健康对照中完成了关于神经源性肠功能障碍（NBD）、布里斯托量表和肠道微生物群的问卷调查，使用16 S rRNA测序。然后，16例中重度NBD评分的SB患者中有11例接受了3个月的TAI。在TAI治疗前后还检查了尿培养的变化。此外，还分析了肠道菌群与布里斯托量表的相关性。与对照组和TAI后相比，SB患者中粪杆菌属、布劳特氏菌属和罗斯拜瑞氏菌属的丰度显著降低，拟杆菌属和罗斯拜瑞氏菌属的丰度显著增加。罗斯拜瑞氏菌的丰度与布里斯托等级呈显著正相关。尽管持续大便失禁，TAI后尿路感染倾向于从82%降至55%（p = 0.082）。丁酸产生菌如罗斯拜瑞氏菌在肠道运动和宿主免疫系统中起调节作用，表明TAI对肠道微生物群的影响。
Recent studies using 16S rRNA-based microbiota profiling have demonstrated dysbiosis of gut microbiota in constipated patients. The aim of this study was to investigate the changes in gut microbiota after transanal irrigation (TAI) in patients with spina bifida (SB). A questionnaire on neurogenic bowel disfunction (NBD), Bristol scale, and gut microbiota using 16S rRNA sequencing were completed in 16 SB patients and 10 healthy controls aged 6–17 years. Then, 11 of 16 SB patients with moderate to severe NBD scores received TAI for 3 months. Changes in urine cultures were also examined before and after the TAI treatments. In addition, correlation of gut microbiota and Bristol scale was analyzed. Significantly decreased abundance in Faecalibacterium, Blautia and Roseburia, and significantly increased abundance in Bacteroides and Roseburia were observed in the SB patients compared with controls and after TAI, respectively. The abundance of Roseburia was significantly correlated positively with Bristol scale. Urinary tract infection tended to decrease from 82% to 55% after TAI (p = 0.082) despite persistent fecal incontinence. Butyrate-producing bacteria such as Roseburia play a regulatory role in the intestinal motility and host immune system, suggesting the effects of TAI on gut microbiota.
登录
查看更多内容
通过元基因组分析揭示与便秘相关的肠道菌群组成和功能。
影响因子：
4.6
作者：
Mancabelli L;Milani C;Lugli GA;Turroni F;Mangifesta M;Viappiani A;Ticinesi A;Nouvenne A;Meschi T;van Sinderen D;Ventura M
通讯作者：
Ventura M
影响因子：
64.8
作者：
Arumugam, Manimozhiyan;Raes, Jeroen;Pelletier, Eric;Le Paslier, Denis;Yamada, Takuji;Mende, Daniel R.;Fernandes, Gabriel R.;Tap, Julien;Bruls, Thomas;Batto, Jean-Michel;Bertalan, Marcelo;Borruel, Natalia;Casellas, Francesc;Fernandez, Leyden;Gautier, Laurent;Hansen, Torben;Hattori, Masahira;Hayashi, Tetsuya;Kleerebezem, Michiel;Kurokawa, Ken;Leclerc, Marion;Levenez, Florence;Manichanh, Chaysavanh;Nielsen, H. Bjorn;Nielsen, Trine;Pons, Nicolas;Poulain, Julie;Qin, Junjie;Sicheritz-Ponten, Thomas;Tims, Sebastian;Torrents, David;Ugarte, Edgardo;Zoetendal, Erwin G.;Wang, Jun;Guarner, Francisco;Pedersen, Oluf;de Vos, Willem M.;Brunak, Soren;Dore, Joel;Weissenbach, Jean;Ehrlich, S. Dusko;Bork, Peer
通讯作者：
Bork, Peer
DOI：
10.1093/dnares/dsw002
发表时间：
2016-04
期刊：
DNA research : an international journal for rapid publication of reports on genes and genomes
影响因子：
--
作者：
Nishijima S;Suda W;Oshima K;Kim SW;Hirose Y;Morita H;Hattori M
通讯作者：
Hattori M
影响因子：
3.7
作者：
Gungor B;Adiguzel E;Gursel I;Yilmaz B;Gursel M
通讯作者：
Gursel M
DOI：
10.2174/1874091x01004010053
发表时间：
2010-05-13
期刊：
The open biochemistry journal
影响因子：
--
作者：
Huda-Faujan N;Abdulamir AS;Fatimah AB;Anas OM;Shuhaimi M;Yazid AM;Loong YY
通讯作者：
Loong YY