Unveiling the gut microbiota composition and functionality associated with constipation through metagenomic analyses.
Unveiling the gut microbiota composition and functionality associated with constipation through metagenomic analyses.
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通过元基因组分析揭示与便秘相关的肠道菌群组成和功能。
DOI：
10.1038/s41598-017-10663-w
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发表时间：
2017-08-29
影响因子：
4.6
通讯作者：
Ventura M
中科院分区：
文献类型：
--
作者：
Mancabelli L;Milani C;Lugli GA;Turroni F;Mangifesta M;Viappiani A;Ticinesi A;Nouvenne A;Meschi T;van Sinderen D;Ventura M
功能性便秘(Function Constipation，FC)是一种在普通人群中发病率较高的胃肠道疾病。FC的确切原因仍不清楚，很可能是多因素的。越来越多的证据表明，肠道微生物区系组成的变化会导致便秘症状。然而，文献中存在许多差异，FC和肠道微生物区系组成之间还没有明确的联系。在这项研究中，我们对68名FC患者的14 7份粪便样本进行了16 S rRNA的微生物图谱分析，并与79名健康受试者(HS)的微生物图谱进行了比较。值得注意的是，FC个体的肠道微生物区系显示出属于杆菌属、Roseburia和Coproñ3的成员被耗尽。此外，使用MiSeq平台通过鸟枪式元基因组学评估了5名FC和5名HS个体的肠道微生物群的代谢能力，表明与FC相比，HS富含碳水化合物、脂肪酸和脂肪代谢的途径。相比之下，与FC对应的微生物群显示出与产氢、产甲烷和甘油降解有关的高丰度基因。已发现的细菌组成和代谢能力的差异可能在FC症状的发展中发挥重要作用。
Functional constipation (FC) is a gastrointestinal disorder with a high prevalence among the general population. The precise causes of FC are still unknown and are most likely multifactorial. Growing evidence indicates that alterations of gut microbiota composition contribute to constipation symptoms. Nevertheless, many discrepancies exist in literature and no clear link between FC and gut microbiota composition has as yet been identified. In this study, we performed 16 S rRNA-based microbial profiling analysis of 147 stool samples from 68 FC individuals and compared their microbial profiles with those of 79 healthy subjects (HS). Notably, the gut microbiota of FC individuals was shown to be depleted of members belonging to Bacteroides, Roseburia and Coprococcus 3. Furthermore, the metabolic capabilities of the gut microbiomes of five FC and five HS individuals were evaluated through shotgun metagenomics using a MiSeq platform, indicating that HS are enriched in pathways involved in carbohydrate, fatty acid and lipid metabolism as compared to FC. In contrast, the microbiomes corresponding to FC were shown to exhibit high abundance of genes involved in hydrogen production, methanogenesis and glycerol degradation. The identified differences in bacterial composition and metabolic capabilities may play an important role in development of FC symptoms.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
DOI：
10.1152/ajpgi.00044.2015
发表时间：
2016-03-15
影响因子：
4.5
作者：
Ringel-Kulka, Tamar;Benson, Andrew K.;Ringel, Yehuda
通讯作者：
Ringel, Yehuda
影响因子：
4.6
作者：
Pozuelo M;Panda S;Santiago A;Mendez S;Accarino A;Santos J;Guarner F;Azpiroz F;Manichanh C
通讯作者：
Manichanh C
DOI：
10.1152/ajpgi.1997.272.1.g147
发表时间：
1997-01-01
影响因子：
4.5
作者：
Coffin, B;Lemann, M;Jian, R
通讯作者：
Jian, R
影响因子：
29.4
作者：
KAMATH, PS;PHILLIPS, SF;ZINSMEISTER, AR
通讯作者：
ZINSMEISTER, AR