Evaluation of 19,460 Wheat Accessions Conserved in the Indian National Genebank to Identify New Sources of Resistance to Rust and Spot Blotch Diseases.
Evaluation of 19,460 Wheat Accessions Conserved in the Indian National Genebank to Identify New Sources of Resistance to Rust and Spot Blotch Diseases.
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DOI：
10.1371/journal.pone.0167702
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Bansal KC
中科院分区：
文献类型：
--
作者：
Kumar S;Archak S;Tyagi RK;Kumar J;Vk V;Jacob SR;Srinivasan K;Radhamani J;Parimalan R;Sivaswamy M;Tyagi S;Yadav M;Kumari J;Deepali;Sharma S;Bhagat I;Meeta M;Bains NS;Chowdhury AK;Saha BC;Bhattacharya PM;Kumari J;Singh MC;Gangwar OP;Prasad P;Bharadwaj SC;Gogoi R;Sharma JB;Gm SK;Saharan MS;Bag M;Roy A;Prasad TV;Sharma RK;Dutta M;Sharma I;Bansal KC
对印度国家基因库中保存的小麦种质资源进行了综合种质评价研究，以确定抗锈病和抗斑枯病的来源。Genebank收集了3个小麦品种：普通小麦（Triticum aestivum）、T. durum和T.在2011-14作物季节期间，在多个疾病热点处顺序筛选双孢属的抗性种质，仅携带抗性种质进行下一步评估。然后对在田间被发现具有抗性的小麦品种进行苗期抗性测定，并使用分子标记进行分析。在初步评估中，在小麦锈病的热点地区惠灵顿（泰米尔纳德邦）筛选了19，460份材料。我们鉴定了4925份具有抗性的材料，并在条锈病热点Gurdaspur（旁遮普）和斑点斑点病热点Cooch Behar（西孟加拉）对这些材料进行了进一步评价。第二轮评价鉴定出498份对多种锈病具有潜在抗性的材料和868份对斑点叶枯病具有潜在抗性的材料。评价抗锈病种质的幼苗抗性对7个有毒的致病型的三个锈病在人工epiphotic条件下确定了137个加入潜在的抗多种锈病。分子分析，以确定不同组合的遗传位点赋予抗叶锈病，秆锈病，条锈病和斑点，使用连锁分子标记，确定了45个小麦种质含有已知的抗锈病基因，以及斑点抗性QTL。本研究中鉴定的抗条锈病种质资源，特别是抗条锈病种质资源，可以通过常规或分子育种方法用于将抗性育种到高产小麦品种的背景中，并有望为国家和全球粮食安全做出贡献。
A comprehensive germplasm evaluation study of wheat accessions conserved in the Indian National Genebank was conducted to identify sources of rust and spot blotch resistance. Genebank accessions comprising three species of wheat–Triticum aestivum, T. durum and T. dicoccum were screened sequentially at multiple disease hotspots, during the 2011–14 crop seasons, carrying only resistant accessions to the next step of evaluation. Wheat accessions which were found to be resistant in the field were then assayed for seedling resistance and profiled using molecular markers. In the primary evaluation, 19,460 accessions were screened at Wellington (Tamil Nadu), a hotspot for wheat rusts. We identified 4925 accessions to be resistant and these were further evaluated at Gurdaspur (Punjab), a hotspot for stripe rust and at Cooch Behar (West Bengal), a hotspot for spot blotch. The second round evaluation identified 498 accessions potentially resistant to multiple rusts and 868 accessions potentially resistant to spot blotch. Evaluation of rust resistant accessions for seedling resistance against seven virulent pathotypes of three rusts under artificial epiphytotic conditions identified 137 accessions potentially resistant to multiple rusts. Molecular analysis to identify different combinations of genetic loci imparting resistance to leaf rust, stem rust, stripe rust and spot blotch using linked molecular markers, identified 45 wheat accessions containing known resistance genes against all three rusts as well as a QTL for spot blotch resistance. The resistant germplasm accessions, particularly against stripe rust, identified in this study can be excellent potential candidates to be employed for breeding resistance into the background of high yielding wheat cultivars through conventional or molecular breeding approaches, and are expected to contribute toward food security at national and global levels.
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影响因子：
2.3
作者：
Bonman, J. Michael;Bockelman, Harold E.;Gironella, Ann Inez N.
通讯作者：
Gironella, Ann Inez N.
影响因子：
2.7
作者：
Elouafi, I;Nachit, MM;Martin, LM
通讯作者：
Martin, LM
影响因子：
1.8
作者：
Eisa, Musaed;Chand, Ramesh;Joshi, Arun K.
通讯作者：
Joshi, Arun K.
影响因子：
5.4
作者：
Hiebert, Colin W.;Thomas, Julian B.;Fox, Stephen L.
通讯作者：
Fox, Stephen L.
DOI：
10.1073/pnas.96.11.5937
发表时间：
1999-05-25
影响因子：
11.1
作者：
Hoisington, D;Khairallah, M;Warburton, M
通讯作者：
Warburton, M